摘要
目的研究血管生成在子宫内膜息肉形成中的作用和宫腔镜子宫内膜息肉切除术(transcervicalresection of polyp,TCRP)对术后子宫内膜再生血管形成的影响。方法子宫内膜息肉患者月经干净后5~7 d行TCRP,取电切下内膜息肉标本25例为子宫内膜息肉组;子宫内膜息肉切除术后1~3个月内,月经干净后3~7 d宫腔镜下取其手术部位内膜20例为TCRP术后组;增殖期子宫内膜25例为对照组。免疫组织化学方法检测各组组织中血管内皮生长因子(VEGF)的表达〔积分光密度(IOD)值〕,显微镜下计数微血管密度(microvessel density,MVD)值。结果子宫内膜息肉组VEGF的表达和MVD值高于对照组和TCRP术后组(P<0.05),而TCRP术后组子宫内膜中VEGF的表达和MVD值与对照组比较差异无统计学意义。结论 TCRP能降低子宫内膜局部的VEGF和微血管生成密度,推测其为TCRP有效治疗子宫内膜息肉的机理之一。
Objective To investigate angiogenesis of endometrium after transcervical resection of endometrial polyp.Methods Twenty five patients were chosen whose final diagnosises are EP.They all conceived the treatment of transcervical resection of polyp in 5-7 days after the end of menstruation.The tissue of EP were resected in the operation were collected as the first group.The second group included 20 patients who conceived the treatment of transcervical resection of polyp,in the 1-3 months after the operation,we collected their endometrium located in the extent of rescection samely in one day of 5-7 days after the ending of their menstruations.25 endometrium in proliferative stage were collected as the third group.expression of vascular endothelial growth factor(VEGF) and CD34 were detected by immunohistochemical methods.We counted the microvessel density(MVD) depending on microscope,and detected integrated optical density(IOD) of VEGF by using special image analysis software(image pro plus 4.5).Results IOD of VEGF expression and MVD of the first group were significantly higher than that of the second and the third group(P>0.05).IOD of VEGF expression and MVD were both same in the second and third group(P>0.05).Conclusion Significance of the angiogenesis in the formation of EP was obvious.Expression of VEGF and MVD could be degraded in someone’s local endometrium after they conceived the treatment of TCRP.These results were the molecular mechanisms how TCRP could cure EP successfully.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期854-857,共4页
Journal of Sichuan University(Medical Sciences)
基金
四川省科技厅重点科技攻关项目(编号:07-kjt-19)资助
