期刊文献+

茶多酚与油菜花粉提取物对前列腺癌细胞生长抑制效果比较研究 被引量:5

Comparation on the Inhibitory Effect of Tea Polyphenols and Rape Pollen Extract on Prostate Cancer Cell
在线阅读 下载PDF
导出
摘要 为研究茶多酚(TP)与油菜花粉提取物对前列腺癌细胞生长抑制效果的差异,应用细胞培养技术、MTT显色试验测定(IC50)法、荧光显微镜观察癌细胞形态变化。结果表明,除花粉醇溶性提取物(B)对前列腺癌细胞没有明显抑制作用外,花粉水溶性提取物(A)及A(B)与TP的等比例混合物均有显著的抑制作用,A、A+TP、B+TP和TP的IC50依次为1470、752、82、45μg/mL,B+TP对PC-3的生长均有抑制作用,且成剂量效应和时间效应关系,经B+TP处理的PC-3癌细胞,用荧光显微镜可以看到癌细胞的凋亡小体。 To study the effect of tea polyphenols (TP) or rape pollen extract (RPE) on the inhibition of PC-3 cell in vitro. MTT assay was used to evaluate the inhibition activity (IC50) on PC-3 proliferation, and fluorescence microscope was used to observe the apotosis cells. Results showed that water-soluble extract of pollen (A), ethanol-soluble pollen extract (B) and TP were proved to be effectively inhibited on the prostate cancer cell PC-3. The IC50 of A, A+TP, B+TP and TP were 1470, 752, 82 μg/mL and 45 μg/mL, ...
出处 《茶叶科学》 CAS CSCD 北大核心 2010年第5期379-383,共5页 Journal of Tea Science
关键词 茶多酚 油菜花粉提取物 前列腺癌 凋亡 tea polyphenols (TP) rape pollen extract (RPE) prostate cancer apotosis
  • 相关文献

参考文献13

二级参考文献18

  • 1侯莉,王强,刘正和.脂溶性花粉提取物的气相色谱-质谱定性分析[J].西北药学杂志,2004,19(4):160-161. 被引量:2
  • 2山原条二 河原有三.前列腺肥大症治疗剂[J].公开特许公报,1992,4(1):1695-1695.
  • 3Kurahashi N,Sasazuki S, Iwasaki M, Inoue M, Tsugane S. Green tea consumption and prostate cancer risk in Japanese men: A prospective study [ J]. AMERICAN JOURNAL OF EPIDEMIOLOGY. 167 ( 1 ), 2008.71.
  • 4Dale W, Vijayakumar S, Lawlor EF, et al. Prostate cancer, race, and socioeconomic status: inadequate adjustment for social factors in assessing racial differences[J]. Prostate, 1996,29(5) : 271.
  • 5D.L.斯佩克特,R.D.戈德曼,L.A.莱因万德.细胞实验指南.(上)[M].北京:科学出版社,2001:105.
  • 6Khan N, Mukhtar H. Multitargeted therapy of cancer by green tea polyphenols [ J ]. CANCER LETTERS. 269 (2), 2008. 269.
  • 7Pfitzenmaier J, Altwein JE. Hormonal therapy in the elderly prostate cancer patient[J].Dtsch Arztebl Int,2009,106(14) :242.
  • 8Pop C, Salvesen GS. Human caspases: activation, specificity, and regulation [J] .J Biol Chem,2009,284(33) :21777.
  • 9Enari M, Sakahira H, Yokoyama H, et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD[J]. Nature, 1998, 391(6662) :43.
  • 10蔡华芳,陈凯,李兰妹,刘雪莉,史红,钱伯初.花粉及醇提物抗前列腺增生与炎症的比较研究[J].中国养蜂,1997,48(4):4-5. 被引量:10

共引文献65

同被引文献52

引证文献5

二级引证文献24

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部