摘要
[目的]观察尿多酸肽(CDA-2)对多发性骨髓瘤细胞RPMI8226抑制增殖和凋亡作用。[方法]应用MTT法,检测CDA-2对多发性骨髓瘤细胞株RPMI8226增殖的影响。通过Hochest33258染色、流式细胞仪(AnnexinV和PI双染色)检测细胞凋亡。[结果]CDA-2明显抑制RPM18226细胞的增殖。CDA-2作用RPM18226细胞增殖24h的半数抑制浓度(IC50)分别为1.64mg/g。采用Hochest33258染色,荧光显微镜下观察CDA-2以2mg/g及4mg/g终浓度作用于RPM1822624h后细胞形态改变,可见明显的凋亡小体出现。AnnexinV-PI双标记法流式细胞仪分析结果显示,RPMI8226细胞2mg/gCDA-2作用6、12、24h后,早期凋亡细胞百分数分别为5.43%、12.02%、29.07%,明显高于空白对照组4.88%。[结论]CDA-2对MM细胞株RP-MI8226有生长抑制作用,可能是通过诱导其发生凋亡而起作用。
[Objective]To investigate the proliferation inhibition and apoptosis-inducing effect of uroacitides(CDA-2)on human multiple myeloma RPMI8226 cells.[Methods]MTT assay was undertaken to observe the growth inhibition of RPMI8226 cells exposed to CDA-2.The apoptosis of RPMI8226 cells were detected by fluorescence staining with hochest33258,flow cytometry(Annexin V and PI staining).[Results]MTT analysis indicated that the proliferation of RPMI8226 cells were inhibited significantly with CDA-2 treatment for 24h.Treatment with different concentrations of CDA-2 for 24h,50% growth inhibition(IC50)at 24h in RPMI8226 was 1.64mg/g.Cell appearances were observed by fluorescence staining with hochest33258 after CDA-2(2mg/g and 4mg/g)for RPMI8226,we could see apoptotic body evidently with fluorescence microscope.After treatment with concentrations of CDA-2(2mg/g)for 6h,12h,24h,RPMI8226 had 5.43%,12.02% and 29.07% apoptotic cells,which was more than that of untreated group(4.88%).[Conclusions]Uroacitides(CDA-2)could inhibit the proliferation and induce apoptosis of human multiple myeloma RPMI8226 cells in vitro.
出处
《浙江中医药大学学报》
CAS
2010年第5期650-651,657,共3页
Journal of Zhejiang Chinese Medical University
