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17β-雌二醇对前脑缺血-再灌注大鼠大脑皮层神经元保护作用及BDNF表达的影响 被引量：4

Neuroprotection of 17β-estradiol on neurons and effects on expression of BDNF in cerebral cortex of rat after forebrain ischemia reperfusion
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摘要 目的观察17β-雌二醇对前脑缺血-再灌注大鼠大脑皮层神经元保护作用及脑源性神经生长因子(BDNF)表达的影响。方法双侧卵巢切除的雌性SD大鼠随机分为四组:生理盐水+假手术组;17β-雌二醇+假手术组;17β-雌二醇+短暂性前脑缺血模型组;生理盐水+短暂性前脑缺血模型组。以低血压联合双侧颈总动脉夹闭10min的方法造前脑缺血模型,只暴露双侧颈总动脉为假手术,17β-雌二醇为药物干预,生理盐水为安慰剂,腹腔注射应用4周。在造模术或假手术后24h处死大鼠,用HE染色检测大脑皮层坏死神经元数目,免疫组织化学染色检测皮层BDNF免疫阳性细胞数,用t检验和两因素析因分析进行统计分析。结果 17β-雌二醇能显著减少大鼠大脑皮层坏死神经元数目,增加BDNF免疫阳性细胞数(p<0.01)。结论 17β-雌二醇对大鼠大脑皮层的神经神经保护作用可能是通过增加相应部位BDNF的表达来实现的。 Objective To observe the neuroprotection of 17β-estradiol on neurons and effects on expression of Brain Derived Neurotrophic Factor in cerebral cortex of rat after forebrain ischemia reperfusion.Methods 40 ovariectomized female SD rats were divided into four groups randomdy: saline + sham;17β-estradiol + sham;17β-estradiol +forebrain ischemia;saline +forebrain ischemia.Forebrain ischemia was induced by bilateral occlusion of the common caroid arteries for ten minutes combined with hypotension.The sham only ...
作者 李正仪 李志伟 郭金涛
机构地区 西安交通大学医学院第一附属医院神经内科
出处 《脑与神经疾病杂志》 2011年第1期24-27,共4页 Journal of Brain and Nervous Diseases
关键词 17Β-雌二醇 前脑缺血 脑源性神经生长因子 大鼠 17β-estradiol Forebrain ischemia Brain-derived neurotrophic factor Rat
分类号 R741 [医药卫生—神经病学与精神病学]
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参考文献14

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二级参考文献21

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共引文献12

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二级引证文献12

脑与神经疾病杂志
2011年 第1期

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