摘要
目的通过构建急性心肌梗死(Acute myocardial infarction,AMI)再灌注兔子模型,观察重组人脑利钠肽(Lyophiluzed Re-combinant Human Brain Natriuretic Peptide,rhBNP)对兔子心肌梗死再灌注损伤的保护作用。方法制备实验性心肌梗死再灌注损伤模型,随机分为 3 组 :A 组 :假手术组、B 组 :模型组、C 组 :rhBNP 组(治疗前组和治疗后组)。测定心肌酶学(血清天冬氨酸氨基转移酶AST、乳酸脱氢酶 LDH、磷酸肌酸激酶 CK)、超氧化物酶(supemxide dismutase,SOD)活性、髓过氧化物酶(myeloperoxidase,MPO)活性、丙二醛(malonaldehyde,MDA)含量以及治疗前后 N 端脑钠素前体(N-terminal pro-brain natriuretic peptide,NT-proBNP)、超敏 C反应蛋白(high-sensitivity CRP,hs-CRP ,hs-CRP)水平变化。结果与 A 组相比,B 组兔子的 SOD 活性明显下降,MDA 及 MPO 含量均明显增加(P<0.05 或 P<0.01);AST、LDH、CK-MB 均显著升高(P<0.01);与 B 组比较,C 组兔子 SOD 活性明显升高,MDA 及 MPO 含量均明显降低(P<0.05);AST、LDH、CK 活性明显降低(P<0.05 或 P<0.01);C 组治疗前与治疗后相比 NT-proBNP、hs-CRP 水平均显著改善(P<0.05),治疗后血清 NT-proBNP、hs-CRP 水平较治疗前下降较为明显(P<0.05)。结论 AMI 再灌注后静脉应用 rhBNP 对缺血梗死再灌注损伤有明显保护作用,对于急性心肌梗死患者来说,重组人脑利钠肽可作为又一种有效的治疗药物。
Object ive The study aimed to investigate the protect ive effect of lyophiluzed recombinant human brain natriuretic peptide against myocardial infarction reperfus ion injury(IRI) in rabbit .Meth ods: I/R model for rabbit was established,thirty rabbits were rondomly into the three gro ups(n=10,respect ively):control group、model group、rhBNP group（Before treatment group and after t reatment group）. myocardial enzymes(level of aspartate trans amin ase、lactate degyd rogenase、creat ine ph osp hokinase)、the su pemx ide dismutas e(SOD)、myeloperox idas (MPO)、 malonaldehyde(MDA) 、N-terminal pro-brain natriuretic pept ide and high-sensit ivity were detected in different groups.Resu lts:the level ofAST、 LDH、CK-MB in gurop B were singni��cantly higher than the group A(P < 0.01), the level of AST、LDH、CK-MB in gurop C were singni��cantly lower than the group B(P < 0.05 or P < 0.01),SOD activity in group B was signi��cantly lower than the group A、the Content of MDA and MPO in group B increases obviously(P < 0.05 or P < 0.01),SOD activi ty in group C was s igni��cantly higher than the group B、the Content ofMDA and MPO in group C lower obviously(P < 0.05); compared with Before treatment group in gurop C,after t reatment ,the level of N-terminal pro-brain natriuretic peptide and high-sensi tivity were highly lower(P < 0.05). Con clusion :There was protective effect of intravenously rhBNP against myocardial IRI in rabbit in vivo, For patients with acutemyocardial infarction with speaking, rhBNP can be used as an effective drug treatment.
出处
《中国分子心脏病学杂志》
CAS
2011年第5期293-296,共4页
Molecular Cardiology of China
基金
青岛市科技局06-2-2-4-nsh-2
关键词
重组人脑利钠肽
心肌梗死再灌注损伤
心肌酶
自由基
疗效
Lyophiluzed Recombinant Human Brain Natriuretic Peptide
Acute myocardial infarction reperfusion injury
Myocardial enzymes
Free radicals
Effectiveness.
