摘要
以西洋参播种苗切断儿为外植体,由发根农杆菌R1601诱导发状根,通过器官发生途径建立高频再生体系,建立西洋参悟性快速繁殖体系。结果表明:诱导西洋参发根脱分化成愈伤组织的最佳培养基为:MS+2,4-D 2.0mg/L+KT 0.5mg/L诱导不定芽增殖和分化的培养基为MS+KT 0.2mg/L+IBA 0.5mg/L诱导生根的培养基为1/2MS+GA_3 5.0mg/L+IBA0.5mg/L+NAA 2.0mg/L。
Took sowed the seeds of Panax quinque folium L.as material,then got the little plants,cut the little plants into segments,deal the segments with Agrobacterium rhizogenes R1601,got the hairy roots of Panax quinque folium L. Lastly,obtained the regenerative plants from hairy roots of Panax quinque folium L.by organogenesis,to satablish the efficient transformation system and regeneration methods for transgenic research.The results showed that the best medium from hairy roots to callus was that MS+2,4-D 2.0 mg/L+KT 0.5 mg/L;The best medium from callus to abventitious bud was that MS+KT 0.2 mg/L+IBA 0.5 mg/L;The best medium for inducting roots was that 1/2MS+ GA_3 5.0 mg/L+IBA 0.5 mg/L+NAA 2.0 mg/L.
出处
《北方园艺》
CAS
北大核心
2011年第10期164-166,共3页
Northern Horticulture
关键词
西洋参
发根农杆菌
R1601转化
植株再生
Panax quinquefolium L.
Agrobacterium rhizogenes
R1601 transformation
plant regeneration
