摘要
目的:探讨海风藤水提物对叠氮钠(NaN3)诱导的PC12细胞损伤的保护作用。方法:将叠氮钠(50mmol.L-1)与PC12细胞共孵育,采用MTT法测定细胞存活力;采用比色法测定细胞乳酸脱氢酶漏出率;采用Fura 2-AM双波长荧光扫描法测定细胞内钙离子内流的浓度。结果:50mmol.L-1叠氮钠与PC12细胞共孵育8h后,细胞存活率明显下降;0.1mg.mL-1海风藤水提物能显著性对抗叠氮钠致神经细胞存活率下降,能显著性降低细胞LDH漏出率,能显著性降低叠氮钠引起的钙离子内流。结论:海风藤对叠氮钠诱导的细胞损伤具有显著性保护作用,可能具有治疗阿尔茨海默病的作用。
Objective:To study the protective effects of the aqueous extract of HaiFengTeng on the PC-12 cell injury induced by NaN3.Methods:PC-12 cells were exposed to sodium azide.The survival rate of the cells was determined by MTT method.The content of LDH was determined by using the assay kit according to the manufacturer's protocol.Intracellular Ca2+was monitored by the method of Fura 2-AM double wavelength fluorescence scanning.Results:The survival rate of the cells was obviously reduced after 8h of incubation with Sodium azide(50mmol·L-1),the aqueous extract of HaiFengTeng(0.1mg·mL-1)could obviously reduce cells injury and LDH induced by NaN3.It could inhibit Ca2+-intake by PC-12 cells.Conclusion:HaiFengTeng has a significantly protective effect on the PC-12 cell injury induced by NaN3 which may have the role in treating Alzheimer's disease.
出处
《中医药学报》
CAS
2011年第3期50-52,共3页
Acta Chinese Medicine and Pharmacology
