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美洲商陆抗病毒蛋白cDNA转化烟草的研究 被引量：3

A Study on Transformation of Pokeweed Antiviral Protein cDNA into Tobacco
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摘要 构建两个美洲商陆抗病毒蛋白(Pokeweed antiviral protein,PAP)基因的植物表达载体pBIPAP和pBIPAPv,通过农杆菌介导的叶盘法转化烟草,经PCR检测证实获得了转基因植株,接种实验显示转基因烟草对TMV具有一定的抗性。 Two plant expression vectors with pokeweed antiviral protein cDNA,pBIPAP and PBIPAPv,were constructed.The pokeweed antiviral protein cDNA were successfully introduced into tobacco by agrobactericum tumefceins. The PAP cDNA was integrated into the genome of tobacco assayed by PCR. Inoculation test showed that the transgenic plants had significant resistance to TMV.
作者 黄冬芬 邓峰 彭世清
机构地区 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室 湖南省安乡县陈家嘴教育办
出处 《华南热带农业大学学报》 2004年第2期1-3,共3页 Journal of South China University of Tropical Agriculture
关键词 美洲商陆抗病毒蛋白 cDNA转化 烟草 转基因植株 phytolacca americana PAP tobcacco transformation TMV
分类号 S572 [农业科学—烟草工业]
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参考文献11

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二级参考文献5

共引文献31

同被引文献44

  • 1谢琪璇,肖銮娟,潘善培,余巍,彭雅林,张春雪.一种快速筛选巴氏毕赤酵母高表达克隆的新方法[J].暨南大学学报（自然科学与医学版）,2004,25(6):713-716. 被引量：3
  • 2赵玉,王锡锋,周广和,李怀方.2个不同缺失型美洲商陆抗病毒蛋白基因在大肠杆菌中的表达[J].植物病理学报,2005,35(3):270-273. 被引量：6
  • 3黄建松,詹金彪,邹媛,冯微宏.美洲商陆抗病毒蛋白-Ⅱ基因的克隆和表达[J].生物工程学报,2006,22(4):592-597. 被引量：4
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华南热带农业大学学报
2004年 第2期

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