摘要
目的:建立羚羊感冒颗粒中牛蒡子苷的含量测定方法。方法:采用Waters2695泵,Waters2487检测器,乙腈-水(25:75)为流动相;检测波长:278nm;流速:0.5mL.min-1;柱温:35℃。结果:进样量0.29888μg～3.7360μg范围内,呈良好的线性关系,r=0.9999(n=6);平均回收率为104.6%,RSD为1.00%(n=6)。结论:本法操作简便,准确,快速,可用于羚羊感冒颗粒的质量控制。
Objective:To develop a method to determine arctiin content in lingyang Ganmao granule by HPLC.Method:Waters2695 pump with Waters 2487 detector was used.The mobile phase was acetonitrile-water(25:75).The detection wavelength was 278 nm.Flow rate was 0.5 mL﹒min-1 and column temperature was set as 35℃.Result:Good linear relationship was found between 0.29888 μg and 3.7360 μg(r=0.9999,n= 6).The average recovery was 104.6% and RSD was 1.00%(n=6).Conclusion:The method is simple,accurate and quick,and is reliable for the quality of Lingyang Ganmao granule.
出处
《中药与临床》
2012年第3期19-21,共3页
Pharmacy and Clinics of Chinese Materia Medica
