摘要
利用黑玉米自交系SDM作为父本,与黄玉米自交系MO17杂交,构建F2∶3群体,对玉米种皮中总酚含量(PCP)和穗轴中的总酚含量(PCC)进行了QTL分析.结果表明:SDM种皮和穗轴中总酚含量均极显著高于MO17,种皮和穗轴中的总酚含量呈极显著正相关.与玉米种皮和穗轴中总酚含量相关的QTL各检测到4个,分布在第4,6,7,9,10共5条染色体上,增效基因均来自父本SDM.其中位于第6和第10染色体上的4个QTL对表型的贡献率最大.控制种皮中总酚含量的2个QTL PCP-6a和PCP-10b分别解释表型变异的13.00%和17.05%,所在的标记区间分别为mmc0523-umc2006和umc1506-bnlg1028,基因作用方式分别为部分显性和加性.控制穗轴中总酚含量的2个QTL PCC-6a和PCC-10a分别解释表型变异的12.20%和21.10%,所在的标记区间分别为umc1014-mmc0523和umc1506-bnlg1028,基因作用方式均为部分显性.位于第6和第10染色体上控制2个性状的QTL可能是一因多效的同一QTL,也可能是紧密连锁的不同QTL.
The F2∶3 population derived from cross of MO17×SDM(YD) is used to identify QTLs of total phenol content of kernel and cob in maize.The result shows that the total phenol content of SDM is significantly higher than that of MO17.There exists highly significant positive correlation between the two traits.Each 4 QTLs for PCP and PCC are identified respectively,which are distributed on chromosome 4,6,7,9 and 10 and most of major QTLs are detected on chromosome 6 and 10.For PCP,two QTLs PCP-6a and PCP-10b explain 13.00% and 17.05% of phenotypic variation,which are detected in the interval mmc0523-umc2006 and umc1506-bnlg1028,with the gene action PD and A,respectively.For PCC,two QTLs PCC-6a and PCC-10a explained 12.20% and 21.10% of phenotypic variation,which are detected in the interval umc1014-mmc0523 and umc1506-bnlg1028 respectively,both with the gene action PD.Maybe these QTLs which are distributed in a same region were closely linked to each other,or they are the same pleiotropic QTL.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第8期106-110,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家大学生创新性试验计划资助项目(091063510)
重庆市重大科技专项资助项目(CSTC2010AA1022)
