摘要
南瓜水提可溶性多糖后产生大量不溶性残渣。以提取可溶性多糖后残留的南瓜渣为底物,采用易降解纤维材料的混合菌种(里氏木霉RUT-C30和白腐真菌5.077 6)深层液体发酵降解南瓜渣的方法制备可溶性多糖。利用Design Expert软件响应面法中心组合设计(Box-Benhnken)优化初始pH(X1),白腐真菌延迟接种时间(X2),接种量(X3)对可溶性多糖得率的影响。结果表明,初始pH4.79,白腐真菌延迟接种时间37.44 h,接种量3.11%时可溶性多糖产率最高。在此条件下进行发酵验证,所得多糖得率预测值为48.633 7%,实际值为47.789 4%,相对误差仅为0.844 3%,实验值与模型预测值吻合度较好。
Large amounts of insoluble residues were produced after water extraction of soluble polysaccharides from pumpkin.Box-Behnken experimental design was applied to study the effect of initial pH(X1),inoculation time delay of white rot fungus(X2) and inoculums(X3) on the production of soluble polysaccharides from pumpkin residue by Trichoderma reesei RUT-C30 and White rot fungi 5.077 6.The conditions of response surface optimization were: initial pH value of 4.79,inoculation time delay of white rot fungus 37.44 h and inoculums 3.11 %.Under these conditions,the yield of polysaccharides could be achieved to 47.789 4 %,compared to predicted value 48.663 7 %.The result showed that experimental value was fit to model prediction,having high inosculating degree and the error was relatively small.
出处
《食品研究与开发》
CAS
北大核心
2012年第6期155-158,共4页
Food Research and Development
基金
国家自然基金(30671469)
关键词
南瓜
多糖
响应面优化
微生物
pumpkin
polysaccharides
response surface methodology
microbiology
