摘要
目的:从福建建瓯黄华山酿酒有限公司高温大曲中分离出89株芽孢杆菌,通过初步筛选鉴定并进行微生物多样性研究。方法:对其16S rDNA进行PCR-RFLP分析和系统发育研究。结果:初步筛选得到的18株芽孢杆菌被HhaⅠ和MspⅠ酶切聚类分为四大组。通过系统发育分析样品中有6株Bacillus subtilis,4株Bacillus cereus,2株Bacillus sonorensis,2株Bacillus licheni-formis,以及Bacillus pumilus、Bacillus oleronius、Bacillus coagulans和Bacillus thuringiensis各1株。结论:研究显示该高温大曲中可培养芽孢杆菌具有微生物多样性。
Objective:89 strains of Bacillus were isolated from Daqu of a Sauce-flavor distillery in Fujian,finally we preliminarily identified and obtained 18 Bacilluses for microbial diversity research.Method:The 16S rDNA of 18 Bacilluses were researched with PCR-RFLP and System growth analysis.Result:They were grouped into four groups by 16S rDNA PCR-RFLP,and then categorized into eight kinds(6 Bacillus subtilis,4 Bacillus cereus,2 Bacillus licheniformis,2 Bacillus sonorensis,1 Bacillus pumilus,1 Bacillus oleronius,1 Bacillus thuringiensis,1 Bacillus coagulan)by 16S rDNA sequence comparison.Conclusion:The cultured Bacilluses from the high temperature Daqu has the microbial diversity
出处
《生物技术》
CAS
CSCD
北大核心
2012年第2期53-58,共6页
Biotechnology
基金
农业部现代农业产业技术体系建设专项资金项目(No.CARS-20-4-4)资助
