摘要
目的:进行竹叶兰干燥根茎中的化学成分和体外抗肿瘤活性研究。方法:乙醇提取,硅胶、SephadexLH-20及ODS柱色谱分离,采用UV,NMR和MS等方法确定化合物的结构。采用MTT法测试化合物对人肝癌细胞Bel-7402和人胃癌细胞BGC-823抗肿瘤药理活性。结果:分得6个化合物,分别为2,7-二羟基-1-(对-羟基苄基)-4-甲氧基-9,10-二氢菲(2,7-di-hydroxy-1-(p-hydroxylbenzyl)-4-methoxy-9,10-dihydrophenanthrene,1),4,7-二羟基-1-(对-羟基苄基)-2-甲氧基-9,10-二氢菲[4,7-dihydroxy-1-(p-hydroxylbenzyl)-2-methoxy-9,10-dihydrophenanthrene,2],3,3'-二羟基-5-甲氧基-联苄(3,3'-dihydroxy-5-me-thoxybibenzyl,3),反式阿魏酸二十四烷酯[(2E)-2-propenoic acid,3-(4-hydroxy-3-methoxyphenyl)-tetracosyl ester,4],反式阿魏酸二十五烷酯[(2E)-2-propenoic acid,3-(4-hydroxy-3-methoxyphenyl)-pentacosyl ester,5],十五烷酸(pentadecyl acid,6)。体外抗肿瘤实验表明,联苄类化合物具有一定的抗肿瘤活性。结论:除化合物3外,另5个化合物均为首次从竹叶兰中分离得到,联苄类化合物均表现出一定的体外细胞毒活性,开环型联苄类化合物3的抗肿瘤活性强于闭环型联苄类化合物1和2。
Objective: To isolate the bibenzyl derivatives from the tuber of Arundina graminifolia and evaluate the anti-tumor activity of these compounds in vitro.Method: The constituents have been extracted by 95% alcohol and then isolated by column chromatography on silica gel and Sephedax LH-20.The structures were determined by UV,IR,NMR and MS spectral analysis.Result: Six constituents have been isolated,and their structures have been established as 2,7-dihydroxy-1-(p-hydroxylbenzyl)-4-methoxy-9,10-dihydrophenanthrene(1),4,7-dihydroxy-1-(p-hydroxylbenzyl)-2-methoxy-9,10-dihydrophenanthrene(2),3,3′-dihydroxy-5-methoxybibenzyl(3),(2E)-2-propenoic acid-3-(4-hydroxy-3-methoxyphenyl)-tetracosyl ester(4),(2E)-2-propenoic acid-3-(4-hydroxy-3-methoxyphenyl)-pentacosyl ester(5) and pentadecyl acid(6),respectively.Conclusion: All compounds except for 3 were isolated from the tuber of A.graminifolia for the first time.Compound 3 with bibenzyl ring opening exhibits stronger anti-tumor activity than that of compounds 1 and 2 with bibenzyl ring closing.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第1期66-70,共5页
China Journal of Chinese Materia Medica
关键词
竹叶兰
联苄
化学成分
抗肿瘤活性
Arundina gramnifolia
bibenzyl derivatives
chemical constituents
anti-tumor activities
