摘要
Eight different inter simple sequence repeat(ISSR) markers were used as tools to investigate genetic variability and population differentiation in the Chinese alligator,Alligator sinensis,in this study. Eleven polymorphic bands(17.2%) out of a total of 64 were generated from 110 individuals in three populations. Analysis of molecular variation showed that most of the genetic variation(98.0%) occurred within the populations. Dendrogram relationship based on Nei’s unbiased genetic diversity illustrated that two breeding populations were genetically closely related. The Nm value of the study was 4.520,suggesting that high levels of gene flow existed and no differentiation appeared in the populations. In a reconstructed Neighbor-Joining tree,the haplotypes coming from the same populations did not gather as a class,suggesting the three populations had no apparent geographic pattern. This study shows that ISSR markers could be well applied as a feasible tool to assess genetic diversity in Chinese alligator individuals.
Eight different inter simple sequence repeat(ISSR) markers were used as tools to investigate genetic variability and population differentiation in the Chinese alligator,Alligator sinensis,in this study. Eleven polymorphic bands(17.2%) out of a total of 64 were generated from 110 individuals in three populations. Analysis of molecular variation showed that most of the genetic variation(98.0%) occurred within the populations. Dendrogram relationship based on Nei's unbiased genetic diversity illustrated that two breeding populations were genetically closely related. The Nm value of the study was 4.520,suggesting that high levels of gene flow existed and no differentiation appeared in the populations. In a reconstructed Neighbor-Joining tree,the haplotypes coming from the same populations did not gather as a class,suggesting the three populations had no apparent geographic pattern. This study shows that ISSR markers could be well applied as a feasible tool to assess genetic diversity in Chinese alligator individuals.
基金
supported by the Excellent Creative Research Team of Animal Biology in Anhui Normal University
the National Natural Science Foundation of China (NSFC, No. 30770312)
the Key Laboratory of Biotic Environment and Ecological Safety of Anhui Province
the Talent Foundation of Fuyang Teachers College
