摘要
利用微生物Absidiasp.R9g产的芦丁-α-鼠李糖苷酶(RhaR)转化芦丁制备异槲皮苷,采用硅胶柱层析法对酶解产物进行分离纯化。结果表明,24.0g芦丁的酶解产物,经分离可得到较纯的异槲皮苷单体13.1g,其得率为49.4%。通过高效液相色谱检测其纯度达到98.3%。
Isoquercitrin was transformation from rutin by rutin-α-rhamnosidase(RhaR) produced with Absidiasp.R9g.Hydrolysate was separated from 24.0g rutin by silica gel column and 13.1g isoquercitrin was obtained.The yield was 49.4%and the purity of the product was 98.3%.
出处
《大连工业大学学报》
CAS
北大核心
2012年第6期399-401,共3页
Journal of Dalian Polytechnic University
基金
辽宁省教育厅创新团队项目(2009T007)
