摘要
目的探讨肾康注射液对腺嘌呤致肾间质纤维化的预防机制。方法将SD大鼠24只随机分为3组,正常对照组,腺嘌呤模型组,肾康注射液干预组,28天后采集血液、尿液标本观察大鼠血尿素氮(BUN)、血肌酐(Scr)和24小时尿量、尿蛋白定量(UTP)水平的变化,留取肾组织,用于HE染色观察肾组织病理改变及免疫组化方法检测肾小管-间质中转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)的表达。结果同模型组相比,干预组可明显降低大鼠Scr、BUN、UTP,降低TGF-β1、CTGF在肾组织中的表达。结论肾康注射液可降低血BUN、Scr、UTP,肾组织CTGF和TGF-β1表达量,延缓肾间质纤维化。
Objective To analyse the interference system of Shenkang injection on adenine-induce renal interstitial fibrosis, Methods Firstly, dividing the 24 SD rat into three groups randomly: the normal group, the adenine model group and the ShenKang injection intervention group; secondly, observing the renal function, changes of the disease feature of the kidney, and changes of the 24h urine, UTP level; and then, surveying the renal TGF-β 1and CTGF by way of immunohis to chemistry staining after 28 days. Results The levels of BUN , Scr , TGF-β1and CTGF expression in intervention group are significantly lower, compared with the model group. Conclusion ShenKang injection can decrease BUN, Scr, TGF-β1 and CTGF expression in nephridial tissue, delay renal interstitial fibrosis.
出处
《中国医学前沿杂志（电子版）》
2012年第9期47-51,共5页
Chinese Journal of the Frontiers of Medical Science(Electronic Version)
关键词
肾间质纤维化
转化生长因子-Β1
结缔组织生长因子
肾康注射液
Renal interstitial fibrosis
Transforming growth factor-β1
Connective tissue growth factor
ShenKang injection
