摘要
采用自行建立和优化的依赖于核酸序列恒温扩增(nucleic acid sequence-based amplification,NASBA)检测体系,对沙门氏菌进行检测。采用沙门氏菌的invA基因为目的片段设计特异性引物,建成可快速检测沙门氏菌的NAS-BA检测法,并进行了特异性和灵敏度试验。结果表明:所建立起的NASBA检测方法,灵敏度为7.1×102cfu/ml,高于普通PCR方法。
A new method,which based on Nucleic Acid Sequence-based Amplification(NASBA) to detect Salmonella of sample,was established.A specific set of primers was synthesized to target the invA gene of Salmonella so as to establish Nucleic Acid Sequence-based Amplification method.Specific and sensitivity were tested.The results shows that the sensitivity of NASBA was 7.1×102 cfu/ml which was higher than the result of PCR method.Detecting Salmonella with NASBA was more specific and sensitive than PCR method and has lower instrumental requirement.
出处
《食品安全质量检测学报》
CAS
2011年第5期248-252,共5页
Journal of Food Safety and Quality
基金
国家质检总局科研项目(2009IK170)资助项目
关键词
沙门氏菌
检测
依赖于核酸序列恒温扩增
salmonella
detection
nucleic acid sequence-based amplification
