摘要
A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H2O2, leading to a limit of detection as low as 10 fM.
A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H2O2, leading to a limit of detection as low as 10 fM.
基金
Supported by the National Natural Science Foundation of China (Grant Nos. 20725516, 20704043, 20873175 & 20805055)
Shanghai Municipal Commission for Science and Technology (Grant Nos. 0752nm021 & 07ZR14136)
Ministry of Sci-ence and Technology (Grant Nos. 2006CB933000, 2007CB936000 & 2007AA06A406)
Ministry of Health (Grant No. 2009ZX10603)
China Post-doctoral Science Foundation and Shanghai Postdoctoral Scientific Program (Grant No. 07R214160).
