摘要
为了提供我国多刺蚁属(Polyrhachis)昆虫的分子系统学资料,我们对9种多刺蚁的mtDNA的Cytb部分序列进行测定和分析。在所得465个位点的序列中,变异位点152个,占32.7%,碱基T、C、A和G的平均含量分别为42.0%、19.6%、29.2%和9.2%,A+T平均含量71.2%。在氨基酸组成上,共编码155个氨基酸。碱基替换主要发生在密码子第三位点,转换频率大于颠换频率,转换主要发生在T与C之间,颠换主要发生在T与A。以弓背蚁属(Camponotus)的黄斑弓背蚁(C. albosparsus)和浅毛弓背蚁(C.albivillosus)为外群,用NJ、MP及贝叶斯推论法构建系统发育树。本研究结果与经典分类结果相符:在9种多刺蚁中,Myrmhopla亚属的双齿多刺蚁(P. dives)独立成为一支,分化较早,位于系统进化树的最底部,最早分化出来;而Myrma亚属的亚毛多刺蚁(P. subpilosa)、梅氏多刺蚁(P. illaudata)、警觉多刺蚁(P. vigilans)以及拟梅氏多刺蚁(P.proxima)聚合成一支,位于系统进化树的最顶端,它们的亲缘关系相近,属于较进化的类群。但是Polyrhachis亚属的叶形多刺蚁(P. lamellidens)和Cyrtomyrma亚属的结多刺蚁(P. rastellata)的进化关系仍需要进一步探讨。
In order to provide molecular systematic data of the ant genus Polyrhachis in China,mtDNA Cytb sequences of 9 species of the genus were analyzed.In the 465 bp fragment of mtDNA Cytb gene,the sequence data revealed considerable variation in 152 nucleotide sites,accounting for 32.7% of the total nucleotide sites,the average contents of T,C,A and G were 42.0%,19.6%,29.2%,and 9.2%,and the contents of A+T was about 71.2%,then 155 amino acids were coded.The substitution of nucleotides mostly occurred at the third position,the frequency of transition was more than that of transversion,transition occurred between T and C,and transversion occurred between T and A.Phylogenetic trees were reconstructed with neighbor-joining(NJ),maximum parsimony(MP) and bayesian inference(BI) methods,using the molecular information of Camponotus albosparsus,C.albivillosus as outgroup both of them belong to Camponotus genus.The results agree with those of classical taxonomy:in nine chosen species,P.dives,which belongs to subgenus Myrmhopla,located at the bottom of the phylogenetic tree,with early differentiated and alone standed.The species include P.subpilosa,P.illaudata,P.vigilans and P.proxima,which all belong to the subgenus Myrma,located at the top of the phylogenetic tree,genetic relationship of them close to each other,and they are the most evolutionary group.But the relationship of P.lamellidens and P.moesta which belong to the subgenus Polyrhachis and Cyrtomyrma respectively still needed to be further discussed are proposed.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第6期1071-1080,共10页
Genomics and Applied Biology
基金
国家自然科学基金项目(30770258)
广西壮族自治区研究生教育创新计划项目(2008106020710M252)共同资助
