摘要
目的探讨血清脂肪酸结合蛋白(intestinalfatty acid binding protein,I-FABP)在脓毒症鼠肠功能障碍评估中的作用及谷氨酰胺对其表达水平的影响。方法以健康SD大鼠腹腔注射大肠杆菌内毒素脂多糖(lipopolysaccharide,LPS)建立脓毒症模型,随机分为3组,对照组不建模,仅给予百普素喂养,模型组建模后给予百普素喂养,谷氨酰胺组建模后同时给予百普素和谷氨酰胺喂养。评估血清I-FABP浓度与肠粘膜损伤指数的相关性及比较3组大鼠血清I-FABP含量。结果血清I-FABP浓度与肠粘膜损伤指数呈正相关(P<0.05);与对照组相比,模型组和谷氨酰胺组血清I-FABP浓度均明显增高(P<0.05);而谷氨酰胺组中血清I-FABP浓度显著低于模型组(P<0.05)。结论血清I-FABP可作为脓毒症肠功能障碍评估的无创性指标;谷氨酰胺能改善脓毒症鼠的肠粘膜的损伤程度。
Objective To investigate the role of intestinal fatty acid binding protein(I-FABP)in evaluating intestinal dysfunction of septic rats and the effect of glutamine on I-FABP expression.Methods Rats were divided into 3 groups,control group were only fed with Peptisorb,model group were fed with Peptisorb after intraperitoneal injection with E.coli endotoxin lipopolysaccharidegiven and glutamine group were added glutamine on basis of model group.The correlation between serum I-FABP level and intestinal mucosa damage index were analyzed and the concentrations of serum I-FABP in each group were observed and compared. Results The serum level of I-FABP in rats were correlated with the Chiu’s score of intestinal mucosa,mucosa thickness and villus length(P<0.05 ).Compared with control group,the concentration of serum I-FABP in model group and glutamine group were significantly increased(P<0.05),but which in glutamine group was lower than that in model group(P<0.05).Conclusions Serum I-FABP could be an non-invasive diagnosis index for evaluating acute intestinal dysfunction in septic rats.In addition,dietary glutamine supplementation may ameliorate sepsis-induced intestinal epithelial injury in rats.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第4期44-46,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
江苏省无锡市科技局立项课题(GSZOON1119)
关键词
肠脂肪酸结合蛋白
急性肠功能障碍
谷氨酰胺
intestinal fatty acid binding protein(I-FABP)
acute intestinal dysfunction
glutamine
