摘要
目的研究蛋白磷酸酶2A的抑癌因子(cancerous inhibitor ofprotein phosphatase 2A,CIP2A)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的差异表达情况及探讨其临床意义及潜在的预后价值。方法应用免疫组织化学和实时定量聚合酶链式反应(polymerase chain reaction,PCR)方法检测60例NSCLC癌组织及其癌旁正常组织2组中CIP2A表达情况,通过检测CIP2AmRNA及蛋白的表达情况,并分析其与临床病理资料和预后因素的关系。结果 CIP2A mRNA肺癌组织比癌旁组织高表达(P<0.01);CIP2A的蛋白在癌组织中的表达明显高于对应的癌旁组织(P<0.01)。CIP2A的表达水平与非小细胞肺癌组织的分化程度及淋巴转移有关(P<0.05),而与性别、年龄、组织学分级、肿瘤大小、TNM(tumor node metastasis)分期无关。结论 CIP2A在非小细胞肺癌组织中高表达,且随着非小细胞肺癌组织分化程度的增高及伴有淋巴结转移而增高。CIP2A及淋巴结转移情况是预后的独立危险因素。
Objective To study the different expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A)in non-small cell lung cancer (NSCLC),investigate its clinical significance and potential prognostic value.Methods Immunohistochemical and real-time polymerase chain reaction (PCR)were used to detect the expressions of CIP2A mRNA and protein in two groups of sixty paired NSCLC specimens and adjacent non-cancer tissues.The relationship between its expression and clinical pathological data and prognostic factors were analysed. Results The expression of CIP2A mRNA in NSCLC specimens was higher than those in adjacent non-cancer tissues(P<0.01 );The expression of CIP2A protein in NSCLC tissues was significantly higher than that in the corresponding adjacent non-cancer tissues (P<0.01 ).The expression of CIP2A was closely correlated with the differentiation and lymph node metastasis of NSCLC tissues (P<0.05 ),while incorrelated with sex,age,pathematology type,tumor size and tumor node metastasis (TNM)stage.Conclusion CIP2A highly expresses in NSCLC,and the expression of CIP2A will increase with the differentiation and lymph node metastasis of NSCLC tissues.The expression of CIP2A and lymph node metastasis can be used as an independent risk factor for the prognosis of patients with NSCLC.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第4期81-84,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
辽宁省科技计划项目(2012225019)
