摘要
粒重是决定水稻产量的三要素之一,是由多基因控制的数量性状。以广陆矮4号为受体,日本晴为供体的119个染色体单片段代换系群体为试验材料,利用SPSS软件分析了粒重与粒形性状之间的相关性,通过单因素方差分析和Dunnett's多重比较,测验单片段代换系与受体亲本广陆矮4号之间粒重的差异,以2年都能检测到的显著差异位点作为稳定表达的QTL。结果表明:千粒质量与粒长、长宽比呈极显著正相关,与粒宽、粒厚相关性不显著;以P≤0.001为阈值,2年都能检测到的千粒质量相关QTL 19个,分布在除第10,12染色体以外的10条染色体上。其中,10个QTL的加性效应表现为增效作用,其加性效应值的变化为0.49～2.74 g,加性效应百分率的变化为2.00%～11.05%;9个QTL加性效应表现为减效,加性效应值的变化为0.60～2.35 g,加性效应百分率的变化为2.40%～9.48%。这些QTL的鉴定,为进一步精细定位或克隆相应QTL奠定了基础。
Grain weight is one of major factors determining rice yield ,which is a typical quantitative trait con-trolled by multiple genes .With Guangluai 4 as recipient and Nipponbare as donor ,a population of 119 chromosome segment substitution lines had been developed .Correlation analysis between grain weight and grain shape by SPSS revealed that 1000-grain weight was significantly and positively correlated with grain length and length -width ratio , while showed no correlation with grain width and thickness .The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett′s test.Nineteen stable QTLs for grain weight were identified over two years.All 19 QTLs were identified on all chromosomes except for chromosome 10 and 12 with a significant level of P≤0.001.Among them,10 QTLs had a positive effect and derived from the Nipponbare allele ,the additive effect of these QTLs ranged from 0.49 g to 2.74 g,the additive effect percentages ranged from 2.00%to 11.05%.Addition-al 9 QTLs had a negative effect and were all derived from Guangluai 4 alleles,the additive effect of these QTLs ranged from 0.60 g to 2.35 g,the additive effect percentages ranged from 2.40%to 9.84%.The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight .
出处
《华北农学报》
CSCD
北大核心
2013年第6期11-17,共7页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31101131)
国家科技支撑计划重大项目(2011BAD16B03)
江苏省科技支撑计划项目(BE2012309)
江苏省自然科学基金项目(BK2011666)
高等学校博士学科点专项科研基金项目(20103250110004)
江苏省农业自主创新资金项目[CX(12)1003]
关键词
水稻
单片段代换系
数量性状
千粒质量
代换作图
Rice
Chromosome single segment substitution lines
Quantitative trait loci
1000-grain weight
Substitution mapping
