摘要
目的研究辐射对转染DNA依赖的蛋白激酶shRNA-(DNA-PK)的卵巢癌Skov3细胞G2的影响。方法培养4个6孔板的Skov3细胞及设计shRNA和shNC载体(对照载体)后进行转染,转染成功后将其分为四个组:shNC组、DNA-PK shRNA组、shNC+照射组、DNA-PK shRNA+照射组,并对照射组分别进行辐射4 h及28 h后采集细胞,采用流式细胞术检测四组Skov3细胞进入G2期的情况。结果 (1)辐射4 h后,四组进入G2期的Skov3细胞所占比例差异具有统计学差异(F=8.65,P<0.01),DNA-PK shRNA+照射组进入G2期的Skov3细胞比例为(18.56±2.79)%,与shNC+辐射组(18.56±1.57)%比较无差异(t=0.00,P=1.00),但明显高于DNAPK shRNA转染组(14.38±1.70)%(t=3.13,P=0.01);(2)辐射28 h后DNA-PK shRNA+照射组进入G2期的Skov3细胞比例是(11.26±3.31)%,与转染shNC+辐射组(11.71±1.28)%比较无差异(t=0.31,P=0.76),但明显高于DNA-PK shRNA转染组(4.42±1.59)%(t=4.56,P<0.01)。结论通过干扰DNA-PK的表达,可使辐射后卵巢癌G2期细胞明显增多,从而提高卵巢癌细胞对于辐射的敏感性。
Objective To investigate the impact on the Skov3 cell of ovarian cancer transfected DNA-dependent protein kinase shRNA in G2 phase after radiation. Methods 4 six-well plates were chosen to culture Skov3 cells and transfected to shRNA and shNC carrier after designing. Then the 4 six-well plates were divided into 4 groups:shNC、DNA-PK shRNA、shNC+radiation and DNA-PK shRNA+radiation. The cells in group shNC+radiation and DNA-PK shRNA+radiation were collected after 4h and 28h radiation. All the Skov3 cells were detected to confirm the proportion of G2 phase though flow cytometry. Results (1) There was significant difference of the proportion of G2 phase in Skov3 cells among the 4 groups after 4h radiation. The proportion of G2 phase of Skov3 cells in group (DNA-PK shRNA+radiation) is (18.56±2.79)%, which was no difference with the group (shNC+radiation) (18.56 ± 1.57)%(t=0.00, P=1.00),but higher than group (DNA-PK shRNA) (14.38 ± 1.70)%(t=3.13,P=0.01);(2)The proportion of Skov3 cells in G2 phase after 28h radiation is(11.26±3.31)%, There was no significant difference with group (shNC+radiation) (t=0.31, P=0.76). But it was higher than the group (DNA-PK shRNA) (4.42±1.59)%(t=4.56, P<0.01). Conclusions It can increased the G2 phase cells of ovarian cancer after radiation significantly though interfere the expression of DNA-PK, so that it can increase the sensitivity of cells in ovarian cancer to radiation.
出处
《妇产与遗传（电子版）》
2013年第4期20-23,共4页
Obstetrics-Gynecology and Genetics (Electronic Edition)
基金
黑龙江省卫生厅项目(2012-489)
关键词
辐射
卵巢肿瘤
蛋白激酶类
Radiation
Ovarian neoplasms
Protein kinases
