摘要
目的采用HPLC法建立苦碟子注射液的指纹图谱。方法以绿原酸、咖啡酸、阿魏酸、木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、菊苣酸混合对照品为参照物;色谱柱Phenomenex Luna C18 4.6mm×250mm,5μm;柱温40℃;检测波长为327nm;以乙腈-0.4%磷酸溶液系统为流动相进行梯度洗脱,流速0.9ml/min。结果 30批供试品液相色谱图中,均检出10个特征峰,相似度不低于0.9。结论为苦碟子注射液的质量评价提供了依据。
Objective The HPLC fingerprints of kudiezizhusheye were established. Methods Use the chlorogenic acid、caf eic acid、ferulic acid、luteolin-7-O-glucoside、luteolin-7-O-β-D-glucuronopyranoside、cichoric acid as the reference substance;a column of Phenomenex Luna C18 4.6mm×250mm,5μm;Column temperature was 40℃,and 327nm as the detection wavelength; determination was conducted by the gradient elution with acetonitrile and 0.4% phosphoric acid, flow rate at 0.9ml?min-1. Results With 30 batches of test articles, al can detected 10 characteristic peak, the similarity index not less than 0.90.Conclusion This method can provide basis estimation of quality for kudiezizhusheye.
出处
《中国药物经济学》
2013年第S1期29-32,共4页
China Journal of Pharmaceutical Economics
