摘要
叶绿体是植物能量转化和光合作用的重要细胞器,也是母本遗传信息的重要载体。叶绿体基因组信息在揭示物种进化、杂交、演变,以及物种鉴定等方面具有重要价值。在其他植物叶绿体基因组研究迅速发展的同时,牧草叶绿体基因组的研究也紧随其后,但针对部分牧草的研究并未深入进行,仅仅是完成了叶绿体基因组全序列的测定。鉴于牧草叶绿体基因组研究的重要性,本文收集整理了近年来的有关资料,从叶绿体DNA的分离、提纯、测序以及牧草叶绿体基因组的研究进展等几个方面归纳了此领域的最新研究成果,旨在使人们进一步了解叶绿体DNA的提纯以及牧草叶绿体基因组的研究现状、今后的发展趋势和应用前景。
Chloroplasts of plant is an important organelle of photosynthesis and energy conversion and it's also an important carrier of female genetic information. Chloroplast genome information is valuable in revealing the evolution of species,hybrids,evolution,species identification and other aspects. With the rapid development of plant chloroplast genome research,researches in herbage chloroplast genome are also executed by leaps and bounds whereas some herbage have not been studied in-depth and only limited in the chloroplast genome sequences. As the importance of chloroplast genome research,the recent relevant information was collected and the latest research progress was summarized in this field from several aspects including isolation of chloroplast DNA,purification,sequencing to understand more about chloroplast DNA purification and the research of status,future trends and prospects of forage chloroplast genome.
出处
《草业科学》
CAS
CSCD
北大核心
2015年第6期978-987,共10页
Pratacultural Science
基金
国家重点基础研究发展计划(973计划)--重要牧草
乡土草抗逆优质高产的生物学基础(2014CB138704)
国家自然科学基金面上项目--紫花苜蓿种子发育分子机理的研究(31272492)
关键词
牧草
叶绿体
基因组
纯化
测序
herbage
chloroplast
genome
purification
sequencing
