摘要
利用简并PCR技术和RACE技术克隆得到了一条洋葱的苯丙氨酸解氨酶(PAL)基因全长cDNA序列。该cDNA序列全长2349 bp,编码长685个氨基酸残基的多肽序列,命名为AcPAL2。Blast分析表明该序列与虎眼万年青Galtonia saundersiae、野蕉Musa balbisiana的相似性均较高。Real-time PCR表达及花青素含量分析表明,红皮洋葱该基因表达量最大,而黄皮和白皮洋葱表达量极低;在红皮洋葱中该基因在膨大初期大量表达,并迅速降低至一定程度后趋于相对平稳表达,且与花青素的积累过程相一致。
A full-length cDNA of phenylalanine ammonia lyase-homologue(AcPAL2)was isolated from onion using degenerate PCR and the RACE(rapid amplification of cDNA ends)method. The full-length cDNA is 2 349 bp long,which encodes a polypeptide of 685 amino acids. Blast analysis indicate that the polypeptide shares a relatively high similarity with Galtonia saundersiae and Musa balbisiana. Real-time PCR analysis demonstrate that Ac PAL2 mRNA express abundantly in red onions,but extremely low in yellow and white onions;AcPAL2 in different bulb swelling stage in red onion has a high expression during the initiation,then express stable after a rapid decrease. And anthocyanin content change also shows the same tendency with AcPAL2 expression.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第3期505-512,共8页
Acta Horticulturae Sinica
基金
国家公益性行业科技项目(20093018)
‘十二五’国家科技支撑计划项目(2012BAD02B00
2011BAD35B07
2012BAD50G01)
北京市科委育种平台三期项目(D111100001311002)
北京市常规育种财政专项(2014-509)
