摘要
以拟康宁木霉(Trichoderma koningiopsis)活菌体为吸附剂探讨了接触时间、初始铜浓度、投菌量、葡萄糖用量、碱浓度等因素对木霉活菌体处理含铜废水效果的影响。结果表明,在初始铜浓度100 mg/L,投菌量3 g/L,预处理碱浓度0.2 mol/L的条件下,木霉活菌体吸附铜效果最佳,吸附量可达5.01 mg/g(湿)。吸附Cu前后的拟康宁木霉菌降解四环素实验结果表明,吸附前后木霉对有机物均有降解效果,吸附过程属活菌体吸附。TEM结果显示,木霉去除铜的机制为表面吸附/络合和胞内富集,FTIR揭示了羟基是活性菌体吸附的主要基团,XRD分析结果证实了羟基与铜结合并以水蓝铜矾[Cu4(SO4)(OH)6H2O]形式络合于菌种细胞壁上,Langmuir吸附等温线较好地拟合了活菌对铜离子的吸附,表明菌体对铜离子主要为均质单层吸附。
Abilities of living cell of Trichoderma koningiopsis were tested as adsorbents for the adsorption of Cu2 +from aqueous solution. Batch adsorption experiments were carried out to study the effects of contact time,the initial concentration,adsorbent dosage,different concentrations of alkali treatment and dosage of glucose on adsorption kinetics mechanism of Cu2 +. The best results( 5. 01 mg / g( wet)) were obtained at 100 mg / L initial copper concentration,3 g / L fungus quantity and 0. 2 mol / L alkali concentration. Tetracycline and organic matter can be degraded by Trichoderma koningiopsis before and after adsorption of Cu2 +,and the Trichoderma koningiopsis kept the life activity during the adsorption process. Results of the TEM indicated that removal mechanisms included the surface adsorption,complexation and cell-bioaccumulation. The FTIR analysis suggested that hydroxyl plays a mian role in biosorption. XRD analysis result showed that copper ions complex on cell walls in the form of posnjakite [Cu4( SO4)( OH)6H2O],it showed that the hydroxyl group is involved in adsorption. And the adsorption data fitted well to the Langmuir adsorption isotherm.
出处
《环境工程学报》
CAS
CSCD
北大核心
2015年第6期2674-2680,共7页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金资助项目(51004053)
福建省自然科学基金资助项目(2013J01162)
中国科学院城市环境与健康重点实验室开放基金(KLUEH201110)
关键词
拟康宁木霉
活菌体
含铜废水
trichoderma koningiopsis
living cell
copper containing wastewater
