摘要
【目的】探讨三明野生蕉Ran基因在低温胁迫响应过程中的作用。【方法】通过RT-PCR与RACE相结合的方法和q RT-PCR克隆Ran基因,并对其在响应低温胁迫和水杨酸处理过程中的表达进行分析。【结果】分离得到6条含有完整开放阅读框的Ran基因c DNA序列,编码的蛋白质与其他植物Ran蛋白高度同源,带有GTP水解结构域、Ran GAP结合结构域和酸性尾巴等典型结构域。q PCR分析结果表明,三明野生蕉Ran基因在根、叶片、花序轴、苞片、花、果皮和果肉中均有表达,在根中表达量最低,在叶片、花和果肉中的表达量较高。此外,低温胁迫和水杨酸处理可显著影响三明野生蕉Ran基因的表达。【结论】三明野生蕉Ran基因与细胞分裂、低温胁迫响应和水杨酸信号转导有关。
【Objective】The objective of the study is to elucidate the mechanism of Ran gene involved in response to low temperature stress.【Method】The c DNA sequence of Ran gene in Yesheng jiao from Sanming was isolated by reverse transcription polymerase chain reaction(RT-PCR) with rapid amplification of cDNA ends(RACE). The expression of this gene in response to low temperature stress and under salicylic acid treatment was determined by real-time quantitative PCR(q RT-PCR).【Result】6 Ran gene cDNA sequences that containing complete open reading frames were isolated from leaves of Sanming yesheng jiao(Musa itinerans Cheesman). All of them encode proteins that show high homology with Ran proteins from other plant species and contain characteristic domains of the Ran proteins including GTP Hydrolysis domain,Ran GAP-binding domain and acidic tail. Results of q PCR analysis indicated that Ragenes were expressed in root,leaf,peduncle,bract,flower,peel and pulp in Sanming yesheng jiao. The lowest m RNA expression was found in root,and the higher expression in leaf,flower and pulp. In addition,the expression levels of Ran genes were affected significantly by low temperature stress and under salicylic acid treatment in wild musa from Sanming.【Conclusion】Ran genes were involved in cell division and responses to low temperature stress and salicylic acid signal transduction in Sanming yesheng jiao.
出处
《果树学报》
CAS
CSCD
北大核心
2015年第1期26-36,共11页
Journal of Fruit Science
基金
国家香蕉产业体系专项资金(CARS-32-11)
福建省重大农业科技平台(2008N2001)
关键词
野生蕉
低温胁迫
Ran基因
基因表达
Sanming yesheng jiao(Musa itineran)s
Low temperature stress
Ran gene
Gene expression
