胶质细胞源性神经营养因子和雌二醇对多巴胺能神经元的协同保护作用被引量：3

Synergetic neuroprotection of GDNF and estradiol on injured dopaminergic neurons

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摘要目的:研究胶质细胞源性神经营养因子(GDNF)和雌二醇(E2)联合使用对6-羟基多巴胺(6-OHDA)所致的黑质多巴胺能神经元损伤的保护作用及其机制.方法:新生2～3 d的雄性SD大鼠行常规中脑脑片培养,6-OHDA诱导损伤,脑片分为正常对照组、溶剂对照组、GDNF组、E2组、GDNF+ E2组、PI3-K特异性阻断剂(wortmannin) +GDNF组、wortmannin+ E2组、wortmannin+ GDNF+ E2组.免疫印迹检测各组脑片中酪氨酸羟化酶(TH)、p-Akt、procaspase-3的表达.结果:在GDNF+ E2组,TH的表达比单用GDNF或E2时显著升高,且procaspase-3的表达也比单用GDNF或E2时增高,差异均有统计学意义;GDNF、E2及GDNF+E2组中Akt磷酸化水平均是升高的,与相应的溶剂对照组相比差异有统计学意义;所有加入wortmannin组与相应的未加wortmannin各组相比,TH和procaspase-3的表达均是降低的,差异有统计学意义.结论:GDNF和E2联合使用对6-OHDA所致的黑质多巴胺能神经元损伤有保护作用,其机制可能是通过PI3K/Akt信号通路介导并且调节Akt下游的procaspase-3的表达变化实现的.

作者刘亚萍王晓舟王萌史子啸徐夏红王婷高殿帅

机构地区徐州医学院形态学实验教学中心徐州医学院机能学实验中心徐州医学院神经生物学实验中心

出处《解剖学杂志》 CAS CSCD 北大核心 2014年第3期334-337,共4页 Chinese Journal of Anatomy

基金国家自然科学基金(30870797)，徐州医学院院课题(2012kJ09)

关键词雌激素多巴胺酪氨酸羟化酶磷酸化-Akt procaspase-3

分类号 R74 [医药卫生—神经病学与精神病学] R33 [医药卫生—人体生理学]

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