摘要
目的:探讨老年慢性阻塞性肺病(chronicobstructivepulmonarydisease,COPD)患者血浆类胰蛋白酶、白细胞介素8(interleukin-8,IL-8)、嗜酸性粒细胞趋化因子水平的相关性及临床意义。方法:老年COPD患者共73例(重度21例,中度21例,轻度31例),正常对照31例。采用双抗体夹心酶联免疫吸附实验,检测血浆类胰蛋白酶,IL-8,嗜酸性粒细胞趋化因子水平。在UniCAP100全自动体外变应原检测仪上进行UniCAPTryptase检测。结果:①老年COPD患者(重、中、轻)急性期血浆类胰蛋白酶水平分别为(7.65±2.79),(6.19±2.50),(4.43±2.08)ng/L,均明显高于对照组(2.86±0.74)ng/L,(t=4.43,5.37,3.34,P均<0.01)。各组急性期血浆IL-8值分别为(99.71±24.05),(45.01±13.33),(26.36±6.00)ng/L,均明显高于对照组(7.66±3.40)ng/L(t=12.32,7.25,6.27,P均<0.01)。各组急性期血浆嗜酸性粒细胞趋化因子值分别为(84.07±17.22),(63.72±10.96),(67.40±22.14)ng/L,明显高于对照组(30.05±10.94)ng/L(t=8.63,6.76,7.71,P均<0.01)。治疗后,重度、中度COPD患者血浆IL-8,嗜酸性粒细胞趋化因子值明显降低(P<0.01);重度、轻度COPD患者血浆类胰蛋白酶值明显降低(P<0.01)。②各组(重、中、轻)急性期外周血嗜中性粒细胞(neutrophils,neu)
AIM:To investigate the correlations between plasma levels of tryptase,interleu kin-8(IL-8) and eotaxin in elderly patients with chronic obstructive pulmonary diseases(COPD) and its clinical significance. METHODS: A total of 73 elderly patiens with COPD(21 severe, 21 moderate and 31 mild) and 31 healthy controls were recruited into the study. IL-8 and eotaxin levels were detected with Sandwich ELISA procedures and tryptase levels were mea sured in the fully automatic extracorporeal allergen detecting instrument UniCAP 100 with antibody against tryptase with UniCAP system. RESULTS: ①At acute attack stage, of elderly patients with (severe,moderate,mi ld)chronic obstructive pulmonary diseases, the plasma levels of tryptase[(7.65 ±2.79), (6.19 ±2.50), (4.43 ±2.08) ng/L, respectively] were obviously higher than those of healthy controls[(2.86 ±0.74) ng/L, (t=4.43,5.37, 3.34,respective ly,P < 0.01)]; the plasma levels of IL-8[(99.71 ±24.05), (45.01 ±13.33), (26. 36 ±6.00) ng/L, respectively]were obviously higher than those of healthy contr ols[(7.66 ±3.40) ng/L,(t=12.32, 7.25,6.27,respectively,P< 0.01)]; the plasma levels of eotaxin[(84.07 ±17.22), (63.72 ±10.96), (67.40±22.14) ng/L, respect ively] were obviously higher than those of healthy controls[(30.05 ±10.94) ng/L ,(t = 8.63,6.76, 7.71,P< 0.01)]. The IL-8 and eotaxin levels in the plasma o f severe and moderate patients were significantly decreased after treatment (P< 0.01). The tryptase levels in the plasma of severe and mild patients were signif icantly decreased after treatment(P< 0.01). ②At acute attack stage, of elderly patients with COPD, the numbers of neutrophils[(13.40±0.90), (8.61±0.73), (6.0 5±0.51) ×109 L-1, respectively] and eosinophils[(0.39 ±0.11),(0.37 ±0.08),( 0.33±0.10)×109 L-1, respectively] in blood were obviously higher than those o f healthy controls[(5.13±0.57),(0.19±0.08) ×109 L-1, respectively, P< 0.01]. After treatment, the elevated numbers of cells were significantly reduced(P< 0. 01).③At acute attack stage, of elderly patients with COPD, there were correlati ons between the blood levels of tryptase, IL-8, eotaxin, neutrophils and eosino phils(P< 0.05). CONCLUSION:COPD is not only a neutrophil associated disease, but also a diseas e to the pathogenesis of which mast cells, eosinophils and their released mediat ors actively contribute.
出处
《中国临床康复》
CAS
CSCD
2004年第15期2854-2856,共3页
Chinese Journal of Clinical Rehabilitation
