摘要
本文对BALB/c小鼠在接种克拉玛依大沙鼠体内的利什曼原虫后一年内发生的组织病理变化作了系统的观察。结果发现该虫能引起接种部位皮肤的充血、水肿、炎细胞浸润、坏死、脓肿或溃疡形成,继而是纤维母细胞、胶原及网状纤维增生并包围虫群。该虫还能在小鼠的尾、左后肢及两前肢足垫、耳、鼻、肝、脾及淋巴结内的病灶中发现。作者认为该虫不但能引起局部皮肤的严重病变,而且还能不断发生远处转移而产生全身性损害,病灶中的宿主细胞反应,胶原及网状纤维含量等的变化,对促成原虫引起病变的局限化有着重要的作用。
The Leishmania from Karamay big gerbils could induce severe histopathological changes of the skin and a series of metastatic lesions in experimentally infected BALB / c mice. The changes of these lesions were further observed in this paper.Twenty BALB/c mice, each inoculated with isolated promastigotes, were killed 0.5-12 months post infection for histopathological examination of the skin and viscera.A half-1 month after infection, a great many macrophages containing leishmania were distributed in the dermis, the subcutaneous tissues showed congestion, edema and eosinophiles infiltration, and the fibroblast cells containing parasites were seen occasionally. Two months post inoculation, focal necrosis and abscess formation occurred in the dermis which contained may ruptured neutrophiles and degenerated leishmania, and the number of lymphocytes surrounding the parasites increased. Besides, a few leishmania were also seen in muscular tissue. Three-4 months after inoculation, the ulcer contained exudates consisting mostly of fibroblasts, many neutrophiles as well as parasites. Four-5 months post infection, plasma cells surrounding the parasites and the number of lymphocytes markedly increased. A few leishmania in the reticular endothelial cells in the liver, spleen and lymphonodes were also seen, but cellular reaction was slight. Five months after infection, necrosis of peripheral nerves in dermis caused by leishmania was observed. Six-7 months post infection, in addition of the above lesions, metastatic lesions of ear occurred, which comprised ulcer and abscess; the cartilage involvement with infiltration of many neutrophiles were also observed. Eight-9 months post inoculation, the number of fibroblast cells at the site of inoculation markedly increased, while concurrently, the number of parasites decreased. 10-12 months post infection, the cutaneous lesions were more or less ameliorated, but both necrosis and ulcer in the tissue were not absorbed completely. The metastatic lesions in the tail, forearm footpad, ear, liver, spleen and lymphonodes were presented continuously.A half-1 month after infection, no changes in content of collagenous and reticular fibers were observed. Two-3 months post inoculation, the content of both kinds of fibers decreased, while in necrotic foci and ulcer of the skin, the collagenous and reticular fibers could not be found. Four-6 months after inoculation, the number of focal necrosis increased and the area of ulcer expanded, the content of fibers the in skin markedly decreased. Seven-9 months post infection, the content of both kinds of fibers around the parasites increased, and some of them spread into necrotic site. Ten months after inoculation, the collagenous and reticular fibers surrounding the leishmania were markedly increased, while the number of parasite markedly decreased. Eleven-12 months post inoculation, although the focal necrosis and ulcer in dermis of BALB / c mice were seen, the number of leishmania markedly decreased or even disappeared, the content of both kinds of fibers was profuse at the site of inoculation.The above results suggested that the Leishmania from Karamay big gerbils might be a parasite with long-term pathogenetic action and metastatic characteristics. The changes of host cellular response and of the content of collagenous and reticular fibers might be the causes contributed to the localization of cutaneous lesions induced by this Leishmania sp.
出处
《地方病通报》
1993年第3期18-23,共6页
Endemic Diseases Bulletin
基金
国家自然科学基金资助项目
关键词
利什曼原虫
致病力
组织病理学
Karamay
Rhombomus opmius
Leishmania
Histopathology
Pathogenicity
Host cellular response
Collagenous fiber
Reticular fiber
