摘要
目的 寻求一种检测一般动物卵母细胞透明带准静态弹性模量的有效途径。方法 根据水牛卵细胞在微管吸吮下的行为 ,作者合理地采用内含无压缩流体固体弹性球壳模型 ,研究水牛卵细胞透明带的力学性质。通过力学分析 ,推导出一个近似计算卵母细胞透明带静态杨氏模量的实验公式。结果 在 2 4℃～ 2 6℃温度下 ,在较大应变范围内测量了 2 0个培养液中成熟无极体卵细胞的杨氏弹性系数 ,数据显示出较好的线弹性特点 ,得到卵细胞透明带的平均弹性系数为 2 .2× 10 4Pa。结论 本研究结果为今后研究动物卵细胞透明带的力学研究提供一种有效的测量方法。
Objective To develop an reliable method to measure Young's modulus of zona pellucida of animal oocytes in a quasi-static statue. Method Elastic sphere model embracing incompresive liquid was adopted to study in vitro mechanical properties of zona pellucida of baffalo oocytes by using micropipette aspiration technique. Based on mechanical analysis, a formula was derived to calculate the Young's modulus of Zona Pellucida in a quasi-static statue. Results A total of 20 mature non-polus oocytes were evaluated within a rather large strain span at temperature of 24～26℃. Their mean Young's modulus E was 2.2×104 Pa, suggesting a good linear elastic property. Conclusion This study provided an effective method to measure elastic property of zona pellucida of animal oocytes.
出处
《医用生物力学》
EI
CAS
CSCD
2004年第2期79-83,共5页
Journal of Medical Biomechanics
基金
国家自然科学重点基金资助 ( 10 3 3 2 0 60 )
关键词
透明带
微管吸吮
球壳模型
杨氏模量
Zona pellucida
Mcropipette aspiration
Spherical shell model
Young's modulus
