摘要
采用高效液相色谱法测定了山楂、决明子颗粒剂中大黄酚的含量.以VP ODS(4.6mm×150mm)为色谱柱,流动相甲醇∶质量分数0.1%磷酸溶液(体积比)为90∶10,检测波长254nm,柱温为室温;大黄酚平均保留时间为7.27min,平均加样回收率为99.33%,RSD为1.87%(n=4),线形范围2.9～29.0μg/mL(r=0.9999,n=6),样品大黄酚的质量分数为0.0557%.该方法准确,灵敏度高,可作为该制剂的质量控制标准.
The effective ingredient in JiangZhi Granules - chrysophanol was determined by high performance liquid chromatography (HPLC). The measurement was conducted by a VP-ODS column(4.6 mm×150 mm) with the mobile phase of methanol/0.1% phosphoric acid(90∶10), at room temperature, and detected with an UV detector at 254 nm. The average retention time of chrysophanol was 7.27 min, the average recovery rate was 99.33%, RSD was(1.87)% (n=4), and a linear range was at 2.9～29.0 μg/mL(r=0.999 9,n=6). The chrysophanol concentration in the sample was 0.055 7%. This method can be used as the product measurement standard with high accuracy and sensitivity.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第4期101-103,共3页
Journal of Wuxi University of Light Industry
