摘要
为了更好地识别川农16的遗传基因特性,为充分利用优异材料99E18改良川农16提供理论依据,应用简单重复序列(SSR)标记、酸性聚丙烯酰胺凝胶电泳(A-PAGE)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对小麦新品种川农16与高抗优质材料99E18间的遗传差异进行了检测与分析。SSR标记检测结果表明川农16与99E18在DNA水平上存在明显差异。A-PAGE分析显示川农16与99E18间至少有12条醇溶蛋白差异带。SDS-PAGE分析表明川农16和99E18的高分子量谷蛋白亚基组成分别为(1,20,5+10)和(1,7+8,5+10)。
The molecular and bio-chemistry genetic diversity between the new cultivar Chuannong16 and (high-quality) material 99E18 was analyzed by SSR markers, APAGE and SDS-PAGE. The results of SSR markers revealed the genetic polymorphism. The analysis of the acid polyacrylamide gel eletrophoresis ((A-PAGE)) indicated that 12 bands were polymorphic. High molecular weight glutenin subunit composition of the two materials were identified by sodium dodeeyl sulphate-polyacrylamid gel electrophoric (SDS-PAGE) and the results showed that 99E18 has 1, 7+8, 5+10 subunit and Chuannong16 has 1, 20, 5+10 subuint.
出处
《麦类作物学报》
CAS
CSCD
2004年第3期25-28,共4页
Journal of Triticeae Crops
基金
十五"国家科技攻关项目(2001BA511B11)。
