摘要
目的探讨ki-67、细胞周期蛋白依赖性激酶4(CDK4)在中耳继发胆脂瘤上皮中的表达,研究它们在胆脂瘤型中耳乳突炎中的作用。方法利用免疫组化的方法,检察32例胆脂瘤上皮和11例正常外耳道皮肤中的ki-67、CDK4蛋白的表达情况。结果ki-67在胆脂瘤上皮和外耳道皮肤中均为阳性表达,阳性细胞率分别为33.2%±13.2%、14.5%±4.9%,两组之间的差异具有显著性(P<O.05);CDK4在胆脂瘤上皮和外耳道皮肤中阳性细胞率分别为35.6%±14.6%、13.6%±5.1%,两组之间的差异具有显著性(P=O.001)。结论ki-67和CDK4在胆脂瘤上皮表达增高。
YE Hui-ping1 , CHEN Qian-mei1,Fan Lu1,Jiang Ke-hui2, LIN Shang-ze1Department of Otolaryngology , Affiliated Hospital of Guiyang Medical College, Guiyang 550004Department of Otolaryngology ,Zunyi Hospital 563001Obejective In this study,we aimed to investigate the role of ki-67?CDK4 in the etiology of middle ear cholesteatoma .Methods The expression of ki-67?CDK4 in the cholesteatoma epithelium(n=32) and in the skin of the external ear canal(n=11) were investigated by an immunohistochemical technique.Results ki-67 was expressed dominantly in the stratum basale and stratum spinosum of cholesteatoma epithelium,but only in the stratum basale of external ear canal skin; CDK4 was expressed mainly in the basal and parabasal cell layers of cholesteatoma epithelium and external ear canal skin. The CDK4-positive cell rate is 35.6%±14.6% and 13.6%±5.1%, respectively. The expression of ki-67 and CDK4 showed significantly difference between two groups of specimens .Conclusion The cholesteatoma epithelium have hyperproliferative ability,which may be contributed to by CDK4.
出处
《中华耳科学杂志》
CSCD
2004年第2期126-128,共3页
Chinese Journal of Otology
