摘要
目的 :比较糖尿病和健康大鼠阴茎细胞凋亡及血流动力学的变化。 方法 :16只糖尿病 (糖尿病组 )和 10只健康Wistar大鼠 (对照组 ) ,注射链脲佐菌素 (STZ ,6 5mg/kg) ,建立糖尿病模型。 8周后处死大鼠 ,测糖化血红蛋白(HbA1c)并取阴茎组织。用原位缺口末端标记 (TUNEL)法和流式细胞术分析阴茎细胞凋亡 ,并测量阴茎动脉血管管径和管壁厚度。 结果 :糖尿病组出现阴茎海绵体细胞凋亡 ,与对照组比较差异有显著性 (P <0 .0 1)。流式细胞术分析糖尿病组大鼠出现凋亡峰 ,与对照组相比差异有显著性 (P <0 .0 5 )。糖尿病组阴茎动脉血管管径变小 ,管壁增厚 ,与照组相比较差异有显著性 (P <0 .0 5 )。 结论 :糖尿病可引起大鼠阴茎细胞凋亡增加 ,导致阴茎动脉的管径变小和动脉的管壁增厚。
Objective: To compare the apoptosis and hemodynamic changes of the penile tissue in diabetic and healthy rats. Methods: Sixteen chronic diabetic and 10 matched normal control rats were injected with a single dose of streptozotocin (STZ) 65 mg/kg and the diabetic model established. Eight weeks later the rats were killed,their haemoglobin Alc(HbAlc) measured,and their penises harvested. TUNEL assay ( in situ nick end-labeling) and flow cytometry were used to analyse the penile cells for apoptosis. The thickness and diameter of the arteries were measured. Results: TUNEL showed apoptosis in the chronic diabetic rats. Flow cytometric analysis showed the apoptotic peak in the penile cells of the chronic diabetic rats when compared with the controls( P < 0.05 ). There was significant difference between the diabetic and healthy rats. Morphological analysis revealed that the thickness of the artery wall increased while the artery diameter decreased in the diabetes group compared with the controls. Conclusion: Diabetes can lead to increasing apoptosis of the penile tissue,cause a decrease in the penile artery diameter and the thickening of the penile artery wall.
出处
《中华男科学杂志》
CAS
CSCD
2004年第6期445-448,共4页
National Journal of Andrology
