摘要
目的 探讨中药紫龙金 (ZLJ)对雄激素非依赖型人前列腺癌细胞系DU 14 5的体外作用及其机制。方法 应用四甲基偶氮唑蓝 (MTT)比色法、软琼脂集落生长试验和流式细胞术测定ZLJ对DU 14 5的增殖抑制、集落生长抑制、周期阻滞和诱导凋亡作用 ,应用逆转录 聚合酶链反应 (RT PCR)和Westernblot方法检测对凋亡相关基因 /蛋白bcl 2和bax ,抑癌基因 /蛋白p16表达的影响。结果 ZLJ具有时间和剂量依赖性增殖抑制、G0 /G1期阻滞和诱导凋亡作用 ,作用 72hIC50 为 0 .42g/L ,ZLJ 0 .1g/L作用 14d对DU 14 5细胞集落生长抑制率为 87.9% ,ZLJ 0 .5 g/L作用 2dG0 /G1期细胞比例从 41.3 0 %增高至 76.70 %。ZLJ可以下调bcl 2基因 /蛋白 ,上调bax和p16基因 /蛋白的表达。 结论 ZLJ可能通过抑制DU 14 5细胞的集落生长、增殖抑制、G0 /G1期阻滞、诱导凋亡、下调bcl 2基因 /蛋白 ,上调bax和 p16基因 /蛋白表达等作用机制发挥抗肿瘤作用。
Objective To investigate the in vitro effects of Chinese herbal medicine Zilongjin (ZLJ) on androgen independent prostate cancer cell line DU 145.Methods Using MTT assay,colony on soft agar and flow cytometry,the effects of ZLJ on anti proliferration,inhibition of clonal growth,cell cycle arrest and apoptosis induction were observed;RT PCR and Western blot methods were used to examine the expression level of apoptosis related genes/proteins bcl 2,bax and tumor suppression gene/protein p16.Results ZLJ could cause apparent anti proliferation effect,induce G 0/G 1 phase arrest and apoptosis induction time dependently and dose dependently on DU 145 cell.Inhibition of cell growth by 50% (IC 50 ) was seen at concentration of ZLJ 0.42 g/L.The clonal growth inhibition rate was 87.9 % after DU 145 cells treated by ZLJ 0.1 g/L for 14 days;The ratio of G 0/G 1 phase cell raised from 41.30% to 76.70% after treatment with ZLJ 0.5 g/L for 2 days.ZLJ also decreased the expression of bcl 2 gene/protein,increased the level of bax and P16 genes/proteins.Conclusion It was seemed that ZLJ has the antitumor action through anti proliferation,inhibition of clonal growth,G 0/G 1 phase arrest,apoptosis induction,decreasing bcl 2 and increasing bax and P16 expression.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第6期720-722,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(39480 0 2 6)
北京市自然科学基金资助项目(7961 0 0 1 )
