摘要
用BTV-HbC3感染人肺癌SPC-A-1细胞,人宫颈癌HeLa细胞,人星型胶质瘤U251细胞,小鼠星形胶质瘤C6细胞及人胚肺HEL细胞后,观察细胞病变效应(CPE);运用透射电镜技术及琼脂双扩散试验检测BTV-HbC3对各种不同肿瘤细胞及人胚肺HEL细胞的感染性;并用RT-PCR技术检测蓝舌病毒的增殖情况。结果显示,BTV-HbC3对正常HEL不感染,但能在不同来源的某些肿瘤细胞中选择性增殖,产生不同程度的细胞病变效应(CPE)及调亡现象,终致肿瘤细胞死亡。其中以人肺癌SPC-A-1细胞对其最为敏感。因此,初步认为BTV-HbC3株能靶向性杀死某些肿瘤细胞,从而为深入开展BTV-HbC3靶向性抗肿瘤的研究提供了第一手实验室依据。
Using BTV-HbC3 to infect tumor cells including human lung cancer SPC-A-1 cells, human cervical carcinoma HeLa cells, human astrocytoma U251 cells, mouse-derived astrocytoma C6 cells and human embryonic lung cells and observe the cytopathic effects(CPE); then using transmission electron microscope and double immunodiffusing test to compare the infectivity of BTV-HbC3 to these cells. Reverse transcription-PCR was used to detect the propagations of BTV-HbC3 in these cells. The results indicated that BTV-HbC3 does not infect normal human embryonic lung cells, but it can selectively replicate in tumor cells and produces different degree of cytopathic effects and can induce apoptosis. Tumor cells were killed in the end. Among all the cells, human lung cancer SPC-A-1 cells have the highest sensitivity to BTV-HbC3. BTV-HbC3 can targetedly kill some kinds of tumor cells. These data provide the laboratory basis for approaching the possib-ility of BTV-HbC3 as an oncolytic virus.
出处
《中国病毒学》
CSCD
2004年第4期349-352,共4页
Virologica Sinica
基金
国家自然科学基金资助项目(30271471)
