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蛋白质工程在改造工业用酶中的应用 被引量：1

Reform of Industrial Enzymes by Protein Engineering WU Hua-chang,DENG Jing,
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摘要 文章概述了蛋白质工程常用策略和用这些策略来改造工业用酶的基本特性(包括稳定性、活性、选择性和表面特性)及其在工业用酶中的应用,并对其发展做了展望。 Strategies for protein engineering and using them to reforming basic properties of industrial enzyme including stability, activity, selectivity, surface properties are summarized. In addition, an application and prospects of industrial enzyme for protein engineering are discussed.
作者 吴华昌 邓静 吴晓莉 韩莎
机构地区 四川理工学院生物工程系四川自贡
出处 《四川轻化工学院学报》 2004年第2期79-82,共4页 Journal of Sichuan Institute of Light Industry and Chemical Technology
关键词 蛋白质 脂肪酶 表面剂活性剂 木聚糖酶 纤维素酶 淀粉酶 protein engineering industrial enzyme rational design directed evolution
分类号 Q814 [生物学—生物工程] TQ93 [化学工程]
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参考文献20

  • 1Hakamada Y, Hatada Y, Ozawa T, et al. Identifation of thermostabilizing residues in a Bacillus alkaline cellulase by construction of chimeras from mesophilic and thermostable enzymes and site-directed mutagenesis[J]. Japan FEMS Microbiology Letters, 2001,
  • 2Kanekoa S, Kunob A, Fujimotod Z, et al. An investigation of the nature and function of module 10 in a family F/10 xylanase FXYN of Streptomyces olivaceoviridis E-86 by module shuffing with the Cex of Cellulomonas fmi and by site-directed mutagenesis[J]. F
  • 3Atsushi K, Daisuke S, Satoshi K, et al. Significant enhancement in the binding ofp-nitrophenyl-L-D-xylobioside by the E128H mutant F/10 xylanase from Streptomyces olivaceoviridis E-86FEBS Letters[J]. 1999,450:299-305.
  • 4Okkels J S, Svendsen A, Patkar S A, et al. Protein engineering of microbial lipase of industrial interest[J].in: F.X.Malcata (Ed.), Engineering of/with Lipases, NATOASI Series, Series E. Applied Sciences, 1995,317: 203-207.
  • 5Yamaguchl S, Takeucht K, Mase Tetal. The consequences of engineering an extra disulfide bond in the Penicillium camembertii mono- and diglyceride specific lipase[J].Prorein Eng., 1996,9:789-795.
  • 6Egmond M R, Vlieg J d, Verheij H M, et al. Strategies and design of mutations in lipases[J]. in: F.X. Malcata (Ed.),Engineering of/with Lipases. Applied Sciences, 1995,317: 193-202.
  • 7Brode P F, Erwin C R, Rauch D S, et al. Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity[J]. Biochemistry, 1996, 36:3162-3169.
  • 8Gloria S R, Gabriel R, Rosa I, et al. Introducing transglycosylation activity in a liquefying K-amylase[J]. FEBS Letters, 1999, 453: 100-106.
  • 9Sierks M, Svennson B. Protein engineering of the relative specificity ofglucoamylase from Aspergillus awamori based on sequence similarities between starch degrading enzymes[J]. Protein Eng.,1994,7:1479-1484.
  • 10Zhang S, Wilson D B[J]. J. Biochem. 1997,57: 101-113.

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四川轻化工学院学报
2004年 第2期

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