摘要
目的 :探讨局灶性脑缺血再灌注时神经元、神经胶质细胞形态变化特点和TNF -α、c -Myc表达的相关性。方法 :采用线栓法大鼠大脑中动脉阻塞复制局部脑缺血再灌注模型 ,缺血 2h分别再灌注 1d、3d、7d ,应用光镜和免疫组化法 ,观察缺血侧额顶叶皮质神经元 ,神经胶质细胞形态变化及TNF -α、c -Myc蛋白表达。结果 :局灶性脑缺血再灌注后 ,同侧额顶叶皮质梗死区神经元、小胶质细胞、星形胶质细胞出现变性、坏死 ,梗死灶周围小胶质细胞和星形胶质细胞增生 ,呈现时间相关性 ,变性、死亡以再灌注 3d最为显著 ,星形胶质细胞和小胶质细胞增生以再灌注 7d最为显著且位于梗死周围区。再灌注后 ,TNF -α、c -Myc阳性细胞表达也显著增加 ,以再灌注 3d最为显著 ,且主要表达于星形胶质细胞、小胶质细胞 ,少量表达于神经元。结论 :脑缺血再灌注后神经元、神经胶质细胞之间在损伤、抗损伤及修复中相互影响 ,而TNF -α。
AIM: To investigate the relationship between morphologic changes in neuron or neuroglial cells and expression of tumor necrosis factor α (TNF-α) and c-Myc in cortex after focal cerebral ischemia/reperfusion in MCAO rats. METHODS: The focal cerebral ischemia/reperfusion model was established by intraluminal thread occlusion of the middle cerebral artery (MCAO). The middle cerebral arteries of rats were occluded for 2 hours and reperfused for 1, 3 and 7 days. Using the techniques of immunohistochemical staining and optical microscopy, the morphologic changes in neuron or neuroglial cells were observed in the cortex of frontal or parietal lobe; the cell types which dynamicaly expressed TNF-α, c-Myc in the different period were also observed. RESULTS: The degeneration or necrosis of neuron or neuroglial cells were observed at the center of infarction, it was very serious at 3 d after reperfussion. Astrocyte and microglial cell proliferation were observed at the broder of infarction. TNF-α and c-Myc positive cells, most of which were astrocytes and microglial cells, increased significantly at 3 d after reperfusion. CONCLUSION: TNF-α and c-Myc may play an important role in the regulation of neuron or neuroglial cells after focal cerebral ischemia with reperfusion. [
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第7期1251-1255,共5页
Chinese Journal of Pathophysiology
基金
安徽省自然科学基金资助项目 (No .0 0 0 0 4 4 116 )
