摘要
从分子水平上研究衰老对大脑的影响有助于揭示机体衰老的分子机理 ,也有助于揭示衰老相关性脑功能异常的发生过程。本研究应用DDRT PCR方法研究衰老相关基因在SAM (Senescence acceleratedmouse)小鼠脑组织中表达的变化情况。在SAMR1TA、SAMP8/Ta、SAMP1 0 /Ta三个品系中 ,通过比较不同鼠龄SAMP1 0 /Ta (2、 4、 1 2、 1 8月龄 )的基因表达情况 ,发现在 4月龄和 1 2月龄分别有一个差异表达片段 ;对不同鼠龄的SAMP8/Ta (2、 4、 1 1月龄 )经差显比较 ,发现在 2月龄和 1 1月龄各有一差异表达片段。在不同品系的比较中发现了 1 6个差异性片段 ,分别属于SAMP1 0 /Ta (3个 )、SAMP8/Ta (6个 )和SAMR1TA (7个 )。测序结果经检索显示 ,它们分别与下列基因转录产物同源 :热休克识别蛋白 70、ATP依赖性线粒体RNA螺旋酶、DleumRNA、小鼠X染色体RP2 3 334C4克隆DNA序列、还原型辅酶Q 细胞色素c还原酶复合物 7 2kD亚单位、 6 0S核糖体蛋白L2 1、FIS、苯基烷基胺钙离子拮抗物结合蛋白、岩藻糖基转移酶 9、胶质细胞源性神经营养因子家族受体α1、内切核酸酶 /逆转录酶、PER1蛋白相关超级融原核蛋白、中心体蛋白CG NAP、转铁蛋白重链基因、巢蛋白 2基因、DNA依赖性蛋白激酶催化亚单位基因
A better understanding of the molecular effects of aging in the brain may help to reveal important aspects of organismal aging, as well as processes that lead to aging related brain dysfunction. In this study, the ageing specific expression genes of the murine cerebrum were investigated by employing differential display reverse transcription polymerase chain reaction (DDRT PCR) in three senescence accelerated mouse (SAM) strains: SAMP8/Ta, SAMP10/Ta and SAMR1TA. Through compared gene expression profile among the age 18, 12, 4, and 2 month of the SAMP10/Ta strain, two differential fragments have been found, which belong to 4 and 12 month individually; and in age 11, 4 and 2 month of the SAMP8/Ta strain, two fragments have been found, which belong to 11 and 2 month respectively. And compared gene expression profile in different strain mice, 16 fragments have been detected, 3, 6 and 7 of them belong to SAMP10/Ta, SAMP8/Ta and SAMR1TA respectively. Sequence results indicate that those fragments are homologous with heat shock protein cognate protein 70,ATP dependent mitochondrial RNA helicase, Dleu2 mRNA, mouse DNA sequence from clone RP23 334C3 on chromosome X, ubiquinol cytochrome c reductase complex (7 2 kD), 60 S ribosomal protein L21, FIS, phenylalkylamine Ca 2+ antagonist (emopamil) binding protein, fucosyltransferase 9, glial cell line derived neurotrophic factor family receptor alpha 1, endonuclease/reverse transcriptase, PER1 interacting protein of the suprachiamatic nucleus homolog, centrosomal protein CG NAP, ferritin heavy chain gene, NID 2 gene, and prkdc gene for DNA dependent protein kinase catalytic subunit.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2004年第4期600-607,共8页
ACTA ZOOLOGICA SINICA
基金
国家重大基础研究规划项目 ( 973 ) (No .2 0 0 0 0 1610 7)
北京灵泰必成医药技术有限公司资助~~
关键词
衰老
小鼠
脑组织
基因
表达
分子机理
Senescence-accelerated mouse, Aging-associated gene expression, Differential display reverse transcription polymerase chain reaction, Cerebral tissue
