摘要
目的:探讨TPA对原代白血病细胞的生长抑制和诱导分化作用。方法:取28例白血病患者原代白血病细胞,分别应用TPA、RA和TPA联合RA进行药物干预培养,用MTT比色法测定各组细胞活力,并观察细胞形态学和组织化学变化。结果:干预培养的原代白血病细胞均出现不同程度的生长抑制。TPA组和TPA+RA组的28例白血病细胞均在12 h^48 h后出现聚集现象。23例ANLL细胞中TPA组19例出现细胞贴壁和胞质丝状突起,TPA+RA组22例出现细胞贴壁和丝状突起。TPA组和TPA+RA组的NAE活性增强。5例ALL均出现细胞聚集反应,NAE染色阴性。结论:TPA可以抑制体外原代急性白血病细胞的生长和增生,并有促使其向单核巨噬细胞分化作用,联合应用RA其抑制效果更明显。
objective:ToinvestigatethedifferentiationinducedbyTPAonhumanprimaryleukemiccells.Methods:Theprimaryleukemiccellsfrom28casesofleukemiawereculturedindifferentculturesystemwithTPA、RAorbothTPAandRA.ThecellvitalitywasassayedbyMTTmethod,atthesametime,themorphologicalandcytochemicalchangeswereobserved.Results:Thecellgrowthofalltheexper-imentalgroupswereinhibitedindifferentdegree.After12h^48hculture,thecongregationofthecellsoc-curredinTPAandTPA+RAgroupsinthe28cases.Inthis23ANLLcases,thephenomenonofcellad-herenceandfilarstick-upswereobservedin19casesofTPAgroupand22casesofTPA+RAgroup.TheNAEactivityofTPAandTPA+RAgroupsincreasedobviously.Inthe5ALLcases,cellcongregationswerealsoobservedandNAEstainwasnegative.Conclusion:TPAcaninhibitthegrowthandproliferationofprimaryacuteleukemiccells,andthisinhibitionenhancedbythecombinationofTPAandRA.
出处
《白血病．淋巴瘤》
CAS
2004年第3期162-164,共3页
Journal of Leukemia & Lymphoma
