摘要
目的 :测定半夏不同生长期总生物碱的含量 ,为确定合理的采收期提供依据。方法 :采用氯仿提取 ,依据酸性染料比色法的原理 ,在 4 17nm波长下测定 ,并作方差分析。结果 :获得良好的线性关系 (r =0 9989)和回收率 ( 10 0 1% ) ,RSD为 2 3%。半夏不同生长期总生物碱含量差异显著 (F =12 789,P <0 0 1)。结论 :测定方法简单快速 ;半夏不同生长期总生物碱含量以全苗期最高 ,每株平均总生物碱产量以集中倒苗期最高 。
Objective:To determine the alkaloids content of Pinellia ternate in different growing time for finding out the best harvest time.Methods:The alkaloid was extracted by chlorolform from Pinellia ternate and tested at 417 nm with the spectroscopy by acid dye. The result was analyzed in One Way ANOVA.Results:The calibration curve of alkaloid was linear(r=0.9989).The recovery rate was perfect (100.1%).The alkaloids content in different growing time had very significant difference (F=12.789,P<0.01).Conclusion:This method is simple and quick.The alkaloids content in whole seedling stage is higher than that in other growing stages,while the mean total alkaloids output per plant is the highest in intensive seedling downfallen stage which is considered as the best harvest time.
出处
《中药材》
CAS
CSCD
北大核心
2004年第7期471-473,共3页
Journal of Chinese Medicinal Materials
基金
四川省教育厅重点科研项目 (2 0 0 1 46)
四川省科技厅杰出青年基金项目 (2 0 0 1 0 1 0 13 0 )
关键词
半夏
总生物碱
含量
动态变化
中药
Pinellia ternate (Thunb.) Breit
Alkaloids
Dynamic change
Growing time
