摘要
目的 观察不同强度恒磁场对过氧化氢 (H2 O2 )作用下人脐静脉内皮细胞 (HUVEC)增殖与凋亡的影响。方法 采用体外培养第 3代的HUVEC ,实验分为 6组 ,即对照组、1 0 0 μmol/LH2 O2 组及H2 O2 +不同强度 (1Gs、5Gs、1 0Gs、50Gs)磁场组。各组细胞于培养及磁场作用 48h后收集标本 ,用MTT比色法测定细胞增殖 ,末端脱氧核糖核酸酶介导的dUTP末端标记法 (TUNEL法 )和流式细胞仪碘化丙啶染色法检测细胞凋亡。结果 1 0 0μmol/LH2 O2 可抑制HUVEC增殖 ,诱导HUVEC凋亡 (P <0 0 5vs对照组 )。 1Gs、5Gs和 1 0Gs恒磁场组细胞增殖显著高于H2 O2 组 (P <0 0 5) ,细胞凋亡显著低于H2 O2 组 (P <0 0 5) ;50Gs恒磁场组细胞增殖和凋亡与H2 O2 组相比无明显差异 (P >0 0 5)。结论 1 0 0 μmol/LH2 O2 可抑制HUVEC增殖并诱导HUVEC凋亡 ;1～ 1 0Gs的恒磁场可强度依赖性拮抗H2 O2 对HUVEC的作用 ,促进HUVEC增殖并抑制HUVEC凋亡。
Objective To investigate effects of constant magnetic field (CMF) on proliferation and apoptosis of human umbilical vein endothelial cells (HUVEC) intervened by hydrogen peroxide (H 2O 2) Methods The third generation of cultured HUVEC were used The cells were divided into six groups: control group, 100 μmol/L H 2O 2group, H 2O 2with one, five, ten or 50 Gs of CMF groups Samples were collected 48 hours later Cell proliferation was measured by MTT chromatometry and apoptosis was determined by propidinm iodide staining on flow cytometry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) Results Hydrogen peroxide of 100 μmol/L inhibited proliferation and induced apoptosis of HUVEC In one, five and ten gauss group, proliferation of cells was significantly higher than that in H 2O 2group ( P <0 05), whereas apoptosis of cells was significantly lower than that in H 2O 2 group ( P <0 05) There was no difference in cell proliferation and apoptosis between 50 Gs group and H 2O 2group ( P >0 05) Conclusions Hydrogen peroxide of 100 μmol/L can inhibit proliferation and induce apoptosis of HUVEC One gauss to ten gauss of CMF can intensity dependently antagonize the effects of hydrogen peroxide on HUVEC, increase the proliferation and decrease apoptosis of HUVEC
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2004年第7期623-625,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目 (30 2 70 396)
关键词
恒磁场
过氧化氢
内皮细胞
增殖
凋亡
Constant magnetic field (CMF)
Hydrogen peroxide
Endothelium
Proliferation
Apoptosis
