摘要
目的:探讨大鼠局灶性脑缺血再灌注早期,皮质和尾壳核中DNA修复酶APE/Ref-1的表达变化情况及其意义。方法:40只SD大鼠单纯随机分为正常组和手术组,其中手术组根据不同时间点分为再灌注6,24和72h组,每组10只动物。通过苏木精-伊红染色方法观察再灌注早期的损伤情况,采用免疫组化和免疫荧光方法检测APE/Ref-1的表达变化。结果:再灌注6,24,72h,皮质损伤区域APE/Ref-1的表达分别为(28.9±8.7),(26.5±10.8),(12.8±6.8)个/视野,与正常组(68.1±12.2)个/视野比,均显著下降(F=2182.92,P<0.01);尾壳核损伤区域APE/Ref-1的表达分别为(21.3±9.8),(20.6±7.6),(13.5±7.3)个/视野,与正常组(72.4±14.7)个/视野比,均显著下降(F=2453.79,P<0.01),并始终维持于较低水平,同时皮质损伤区域细胞胞浆内出现APE/Ref-1的异常表达。结论:再灌注早期DNA修复酶APE/Ref-1表达水平的降低及其在胞浆中的滞留,提示损伤区域DNA修复功能下降,并可能因此促进DNA损伤的积累和发展。
AIM:To explore the changes and significance of the expression of DNA repair en zyme apurinic/apyrinidinic endonuclease/ redox factor 1 (APE/Ref-1) in cortex a nd tail putamen at the early stage of cerebral ischemia/ reperfusion in rats. METHODS:Forty SD rats were randomly divided into normal group and operated gro up,and the operated group was subdivided into reperfusion 6,24 and 72 hours grou ps according to the different time points, and there were 10 rats in each groups . Early damage changes were observed through HE staining, and changes of APE/Ref -1 expression were detected through immunohistochemical and immunofluorescent m ethods. RESULTS:The expressions of APE/Ref-1 in the damaged regions of cortex at 6,24 and 72 hours after reperfusion were (28.9±8.7),(26.5±10.8) and (12.8±6.8) pe r visual field,respectively,significantly decreased compared with that in the no rmal group [(68.1±12.2) per visual field](F=2 182.92,P< 0.01);and those in the damaged regions of tail putamen were(21.3±9.8),(20.6±7.6)and(13.5±7.3) per vi sual field, significantly decreased compared with that in the normal group [(72. 4±14.7) per visual field](F=2 453.97,P< 0.01),which was always kept in a low le vel,and meanwhile, the abnormal expression of APE/Ref-1 was observed in the cyt oplasm of injured cortex. CONCLUSION:The reduced level of DNA repair enzyme APE/Ref-1 and its retention in the cytoplasm at early stage of reperfusion demonstrate that DNA repairing f unction is weakened,which may play an important role in accelerating DNA damages .
出处
《中国临床康复》
CSCD
2004年第22期4486-4487,i002,共3页
Chinese Journal of Clinical Rehabilitation
