摘要
背景肿瘤坏死因子(TNF)在体外能诱导某些正常细胞和大多数肿瘤细胞株(包括Hela细胞株)凋亡,其凋亡发生机制与细胞周期的关系中尚有许多问题需要探讨。目的研究细胞周期时相对TNF诱导Hela细胞凋亡的影响。设计非随机非对照实验研究。地点和对象实验在广东医学院生物化学与分子生物学研究所完成,对象为重组人肿瘤坏死因子-α(rhTNF-α,Sigma产品),Hela细胞株引自中国科学院上海细胞生物研究所。干预通过胸腺嘧啶核苷酸(TdR)阻断法阻滞Hela细胞的细胞周期,采用MTT法、流式细胞术和荧光染色分析TdR阻滞细胞和周期化的Hela细胞对TNF诱导凋亡的敏感性。主要观察指标TNF诱导TdR阻滞和周期化Hela细胞凋亡数。结果TdR阻滞细胞周期较周期化的Hela细胞对TNF诱导凋亡的敏感性降低。结论TNF诱导Hela细胞凋亡与细胞周期有关。
BACKGROUND:Previous researches had confirmed that TNF in vitro could induce apoptosis in some of normal cells and most tumor cells including Hela cell.Some problems between mechnism of apoptosis and relationship of cell cycle should be explored.OBJECTIVE:To study the effect of cell cycle in Hela cells on apoptosis induced by tumor necrosis factor.DESIGN:Nonrandomised uncontrolled study was adopted.SETTING and PARTICIPANTS:The study was completed in the Department of Biochemistry and Molecular Biology of Guangdong Medicine College.Recombinated human TNF α(rhTNF α)was provided by Sigma Co.Hela cells were derived from Cellular Biological Institute of Science Academy of China in Shanghai.INTERVENTIONS:Hela cells was arrested by cell cycle blocking drug [(3H)Thymidine Deoxyribose(TdR)].Hela cells in arresting and cycling were treated with tumor necrosis factor. Cell apoptosis was evaluated by MTT assay, flow cytometry and fluorescent staining.MAIN OUTCOME MEASURE:Blocks of cell cycle by TNF and the apoptosis number of Hela cells were measured.RESULTS:Hela cells by cell cycle arrest became lower sensitive to TNF induced apoptosis.CONCLUSION: Hela cells to apoptosis induced by tumor necrosis factor is related to cell cycle.
出处
《中国临床康复》
CSCD
2004年第23期4918-4919,F003,共3页
Chinese Journal of Clinical Rehabilitation
基金
广东省重点学科经费资助项目(9608)~~
