摘要
甘薯茎尖分生组织培养与快速繁殖技术研究课题己进行3a,筛选出最佳培养基为MS+6-BA1.0mg/L+NAA0.1～0.2mg/L+3%蔗糖+琼脂6.0g/L;最适宜培养温度为26～30℃;症状学诊断法、指示植物鉴定法进行病毒检测,平均脱毒率达83.7%.脱毒试管苗切段快速增殖,繁殖系数为5n,繁殖速度以几何级数增长,一定时间内可大量繁殖脱毒苗.脱毒苗移栽大田进行产量比较,平均增产率达37.8%.
The three-year research on stem-apex meristem culture and fast reproduction technology of sweet potato selected out MS+6-BA 1.0mg/L+NAA 0.1~0.2mg/L+3%sucrose+agar6.0g/L and 26~30℃ respectively as the preferential medium and appropriate temperature range. And the average devirus rate was 83.7% based on the semiological diagnostic and plant indicator identification. The reproduction coefficient for the fast propagation with cut test-tube plantlets was 5 n, the reproduction speed increased at a geometric progression and large-scale seedlings could be multiplied in a short time. The virus-free seedlings transplanted in fields reached a yield increase of 37.8%.
出处
《中国农学通报》
CSCD
2004年第4期33-35,共3页
Chinese Agricultural Science Bulletin
基金
杨凌职业技术学院科研基金资助项目
