摘要
目的 研究细胞外信号调节蛋白在青石棉致肺部疾病的作用。方法 用Western免疫印迹、免疫沉淀等对石棉刺激A5 4 9细胞后细胞外信号调节蛋白及其下游Elk1蛋白磷酸化等进行了研究。结果 石棉刺激细胞后 ,磷酸化ERK1 2、Elk1等高表达 ,差异有显著性 (P <0 0 5 )。结论 ERK1 2。
Objective To investigate the role of extracelluar signal regulated kinase (ERK) 1/2 and Elk1 in pulmonary disease induced by crocidolite asbestos fiber. Method Western blotting and Immunoprecipitation were used to detect the expression of phosphorylated ERK1/2 and Elk1 in human bronchial airway A549 cell line stimulated by crocidolite. Results The expression of phosphorylated ERK1/2 and Elk1 were striking higher than those of the control, the differences were significant (P<0.05). Conclusion Phosphorylated ERK1/2 and Elk1 probably involved in the process of the diseases induced by crocidolite.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第4期398-399,共2页
Journal of Hygiene Research
基金
美国国家环境保护局国立卫生与环境效应研究所临床医学部
北卡罗来那大学环境医学
哮喘和肺生物学研究中心联合资助 (CR 82 592 2 )
2 1 1重点学科研究项目肿瘤与生物工程子项目
