摘要
目的 :探讨整合素 β3 反义寡核苷酸 (ASODN)活体转染假孕小鼠子宫内膜抗早孕的可能性。方法 :将整合素 β3 反义寡核苷酸注入假孕 1d小鼠子宫 ,同时同侧输卵管植入 2细胞期受精卵 1 0枚 ,小鼠正常饲养 ,5d天取小鼠子宫内膜进行WesternBlotting检测整合素 β3 蛋白表达情况 ;1 9d进行小鼠子代出生率评估。结果 :①WesternBlotting检测 5d天小鼠子宫内膜整合素 β3 蛋白强表达 ,1 0 pmol整合素 β3 反义寡核苷酸转染假孕小鼠子宫内膜后5d天小鼠子宫内膜整合素 β3 蛋白减弱表达 ,1 0 0pmol整合素 β3 反义寡核苷酸转染时弱表达 ,单纯脂质体轻度影响其表达 ;② 1 9d进行小鼠子代出生率评估 ,受孕鼠转染整合素 β3 反义寡核苷酸后子代小鼠出生抑制率为 85 %。结论 :整合素 β3 反义寡核苷酸可能整合到假孕小鼠子宫内膜抑制其蛋白表达 ,阻止胚胎着床。由于例数较少 ,其整合素 β3
Objective:To investigate the possibility of resisting early pregnance when the endometrosis of pseudo-pregnancy mice vivo-transfected by integrin-β_3 ASODN.Method:Infused integrin-β_3 to the endometriosis of pseudo-pregnancy mice dl,at the same tima,the fertilized eggs be implantated to the homolateral duct.The mice be bred normally.Five days later,detect the expression of integrin-β_3 albumen in the endometriosis and estimated the natality of filizl generation after 19 days.Result:①There is strong expression of integrin-β_3 in mice,s endometriosis detected by Western Blotting.When pseudo-pregnancy mice transfected by l0pmol integrin-β_3 ASODN,the expression of integrin-β_3 in the indometriosis was weakened meanutile no expression when 100pmol did.Simple lipin didn't affect the expression.②Estimated the natality of filial generation 19 days later.The inhibition rate of filial generation is 85% when transfected by integrin-β_3 ASODN.Conclusions:Integrin-β_3 ASODN could be integrated to the endometriosis of pseudo-pregnancy mice,and inhibited the expression of its albumen,then prevent the embryo,s implantation.
出处
《赣南医学院学报》
2004年第3期246-249,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
