摘要
背景通过口服抗原诱导对自身抗原的耐受,已被大量用于自身免疫病的实验研究中,为自身免疫病的治疗带来了一线曙光。目的研究口服鸡Ⅱ型胶原对大鼠实验性关节炎的治疗作用及其机制。设计随机对照实验研究。地点和对象实验在四川大学基础医学与法医学院免疫教研室完成。50只雄性SD大鼠(合格证号川实动管质第99-32号,四川抗菌素工业研究所动物中心提供),2～3月龄,大鼠级别为二级,在清洁系统下喂养。干预SD大鼠(n=40)左后足皮内注射完全弗氏佐剂,每只0.1mL,建立大鼠佐剂型关节炎动物模型,按随机数字表法分成阳性治疗对照组、低剂量治疗组、中剂量治疗组和高剂量治疗组,每组10只。通过胃管给药方式分别给予泼尼松25mg/kg、高(25mg/kg)、中(0.01mg/kg)、低剂量(0.03mg/kg)的鸡Ⅱ型胶原,连续用药2周。正常对照组和关节炎模型对照组(各10只)只给予醋酸溶液,连续用药2周。主要观察指标①大鼠关节病变的发生和严重程度。②大鼠外周血T细胞亚群的变化。③大鼠外周血TNF-α水平的变化。结果成功建立了大鼠佐剂型关节炎实验动物模型,口服高、中、低剂量鸡Ⅱ型胶原的大鼠,与关节炎模型对照组比较,关节病变明显得到改善,其中高剂量组的作用尤为显著。高剂量治疗组的大鼠外周血CD3+T细胞、CD4+T细胞和CD8+T细胞水平分别?
BACKGROUND:Oral antigen administration has been extensive used experimentally to induce tolerance of autoantigens,which shed light on a potential therapeutic strategy for autoimmune diseases.OBJECTIVE:To investigate the suppressive effect of oral chick type Ⅱcollagen( CⅡ) treatment on rat adjuvant induced arthritis and explore the mechanism.DESIGN:A randomized controlled trial.SETTING and PARTICIPANTS:The research was conducted in the Department of Immunology,College of Basic Medical Science and Forensic Medicine,Sichuan University.A total of 50 male SD rats of grade two aged 8 to 12 months were obtained from Sichuan Institute of Antibiotic Industry,and fed in clean environment.INTERVENTIONS:Forty rats received intradermal injection with 0.1 mL Freunds complete adjuvant in the left hindfoot to build an animal model of adjuvant induced arthritis.The rats were then randomly divided into positive treatment control group and 3 CⅡgroups of low,intermediate and high doses with 10 in each group,treated via a gastric tube with 25 mg/kg prednisone and 0.01,0.03 and 25 mg/kg CⅡrespectively for 2 consecutive weeks.Another 10 normal rats and another 10 rat models served as the normal control and model groups,respectively, given acetic acid solution instead for 2 weeks.MAIN OUTCOME MEASURES:The occurrence and severity of arthritic lesion and the changes of T cell subsets and tumor necrosis factor α(TNF α) concentration in peripheral blood were observed in the rats.RESULTS:Feeding of CⅡfor 2 weeks could ameliorated adjuvant induced arthritis,especially in the high dose group.The percentages of peripheral CD3+, CD4+and CD8+T cell subsets in high dose CⅡgroup were(84.35±3.92) %,(34.37±5.72) %and(31.89±9.71)%respectively,significantly different from those of the positive control group(P< 0.05-0.01).The serum TNF αlevel also decreased significantly to(1 485.0±119.3) ng/L as compared with(1 785.4±119.0) ng/L in the positive control group(P< 0.05).CONCLUSION:Oral administration of chick CⅡcan suppress adjuvant induced arthritis in rats by inducing oral tolerance.
出处
《中国临床康复》
CAS
CSCD
2004年第24期5162-5163,共2页
Chinese Journal of Clinical Rehabilitation
